Anti-HBc Total (IgG/IgM) Enzyme Immunoassay (EIA)
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Reference Details
Detection of total antibody (IgG and IgM) to the Hepatitis B virus core antigen marker by enzyme immunoassay (EIA).
- Hepatitis B
Serum or plasma sample. Minimum volume required for serum or plasma – 0.5 mL.
Collect blood in serum separator tubes (SST) or EDTA tubes.
Store samples refrigerated or frozen until shipped for testing. Ship frozen samples on dry ice, and refrigerated samples on wet ice
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
Suspected Hepatitis B infection.
Completed Viral Hepatitis and Bloodborne Pathogens requisition including sender name, address and telephone number. Patient name or identifier (referring specimen lab #), date of birth, suspected exposure, test(s) requested. Type of specimen and date collected. If possible, include the clinical history and lab results that have already been done at local or provincial laboratories.
N/A
Automated EIA assay (Roche Elecsys). Anti-HBc is used typically as a qualitative serological marker of ongoing or past infection.
15 calendar days.
- Schmidt M, Jimenez A, Mühlbacher A, Oota S, Blanco L, Sakuldamrongpanich T, Schennach H. Seifried E. Head-to_head comparison between two screening systems for HBsAg, anti-HBc, anti-HCV and HIV combination immunoassays in an international, multicentre evaluation study. Vox Sang 2015 109:114-121.
- Public Health Agency of Canada (2013) Primary Care Management of Hepatitis B – Quick Reference. Authors: Andonov, A. Ling, R., Baril, J., Myers, R., Brubacher, C., Osiowy, C., Butler, G., Pritchard, L., Gale-Rowe, M., Verkoeyen, J., Heathcote, J., Yim, C., and Latham-Carmanico, C. http://publications.gc.ca/pub?id=439952&sl=0.