Molecular Detection of Hepatitis B virus Precore/ Basal Core Promoter Mutants<<Return to Laboratory
Molecular detection of Hepatitis B virus precore and/or basal core promoter mutants by sequence analysis.
- Hepatitis B
Serum or plasma sample. Minimum volume required for serum or plasma – 1.0 mL.
Collect blood in serum separator tubes (SST) or EDTA tubes.
Store samples frozen until shipped for testing. Ship frozen on dry ice.
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
Suspected Hepatitis B infection. Serology results must show markers to the Hepatitis B virus and HBV DNA results must be positive (i.e, must have detectable viral load).
Completed Viral Hepatitis and Bloodborne Pathogens requisition including sender name, address and telephone number. Patient name or identifier (referring specimen lab #), date of birth, suspected exposure, test(s) requested. Type of specimen and date collected. If possible, include the clinical history and lab results (particularly HBeAg/eAb test results) that have already been done at local or provincial laboratories.
Conventional PCR followed by sequence analysis and alignment with reference sequences. The presence of the precore (G1896A) or basal core promoter (A1762T/G1764A) mutations are associated with loss or reduction of HBeAg expression in patients with ongoing chronic hepatitis.
19 calendar days.
- Jammeh S, Tavner F, Watson R, et al. Effect of basal core promoter and pre-core mutations on hepatitis B virus replication. J Gen Virol 2008 89:901-909.
- Alidjinou EK, Bocket L, Pigot V, et al. Sanger sequencing versus INNO-LiPA HBV PreCore assay for routine detection of precore and basal core promoter mutations in hepatitis virus B chronically infected patients. Diagn Microbiol Infect Dis 2018 90:277-279.