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Molecular Detection of Hepatitis B virus Precore/ Basal Core Promoter Mutants

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Requisition Forms

Reference Details

Description:

Molecular detection of Hepatitis B virus precore and/or basal core promoter mutants by sequence analysis.

Test Category:
Molecular Detection
Pathogen:
Hepatitis B virus (HBV)
Illnesses and Diseases:
  • Hepatitis B
Specimen:

Serum or plasma sample. Minimum volume required for serum or plasma – 1.0 mL.

Collection Method:

Collect blood in serum separator tubes (SST) or EDTA tubes.

Specimen Processing, Storage and Shipping:

Store samples frozen until shipped for testing. Ship frozen on dry ice.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

 

Patient Criteria:

Suspected Hepatitis B infection. Serology results must show markers to the Hepatitis B virus and HBV DNA results must be positive (i.e, must have detectable viral load).

Accompanying Documentation:

Completed Viral Hepatitis and Bloodborne Pathogens requisition including sender name, address and telephone number.  Patient name or identifier (referring specimen lab #), date of birth, suspected exposure, test(s) requested. Type of specimen and date collected.  If possible, include the clinical history and lab results (particularly HBeAg/eAb test results) that have already been done at local or provincial laboratories.

Comments:

N/A

Methods and Interpretation of Results:

Conventional PCR followed by sequence analysis and alignment with reference sequences. The presence of the precore (G1896A) or basal core promoter (A1762T/G1764A) mutations are associated with loss or reduction of HBeAg expression in patients with ongoing chronic hepatitis.

Turnaround Time:

19 calendar days.

Contact:
Phone #: (204) 789-6061
Fax: (204) 318-2222
References:
  1. Jammeh S, Tavner F, Watson R, et al. Effect of basal core promoter and pre-core mutations on hepatitis B virus replication. J Gen Virol 2008 89:901-909.
  2. Alidjinou EK, Bocket L, Pigot V, et al. Sanger sequencing versus INNO-LiPA HBV PreCore assay for routine detection of precore and basal core promoter mutations in hepatitis virus B chronically infected patients. Diagn Microbiol Infect Dis 2018 90:277-279.