Molecular Detection of Hepatitis B virus Precore/ Basal Core Promoter Mutants

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Requisition Forms

Reference Details


Molecular detection of Hepatitis B virus precore and/or basal core promoter mutants by line probe hybridization assay or by sequence analysis.

Test Category:
Molecular Detection
Hepatitis B virus (HBV)
Illnesses and Diseases:
  • Hepatitis B

Serum or plasma sample. Minimum volume required for serum or plasma – 1.0 mL.

Collection Method:

Collect blood in serum separator tubes (SST) or EDTA tubes.

Specimen Processing, Storage and Shipping:

Store samples frozen until shipped for testing. Ship frozen on dry ice.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

Patient Criteria:

Suspected Hepatitis B infection. Serology results must show markers to the Hepatitis B virus and HBV DNA results must be positive (i.e, must have detectable viral load).

Accompanying Documentation:

Completed Viral Hepatitis and Bloodborne Pathogens requisition including sender name, address and telephone number.  Patient name or identifier (referring specimen lab #), date of birth, suspected exposure, test(s) requested. Type of specimen and date collected.  If possible, include the clinical history and lab results (particularly HBeAg/eAb test results) that have already been done at local or provincial laboratories.



Methods and Interpretation of Results:

Conventional PCR followed by 1) line probe hybridization assay, in which reactivity to 1896A (precore) or 1762T/1764A (basal core promoter) probes indicates a mutation, or 2) sequence analysis.

Turnaround Time:

19 calendar days.

Phone: (204) 789-6061; HCV (204) 789-5063
Fax: (204) 789-2082
  1. Olivero A, Ciancio A, Abate ML, Gaia S, Smedile A, and Rizzetto M. Performance of sequence analysis, INNO-LiPA line probe assays and AFFIGENE assays in the detection of hepatitis B virus polymerase and precore/core promoter mutations. J Viral Hepatitis 2006 13:355-362.
  2. Jammeh S, Tavner F, Watson R, et al. Effect of basal core promoter and pre-core mutations on hepatitis B virus replication. J Gen Virol 2008 89:901-909.