Molecular Detection of Hepatitis B virus Precore/ Basal Core Promoter Mutants<<Return to Laboratory
Molecular detection of Hepatitis B virus precore and/or basal core promoter mutants by line probe hybridization assay or by sequence analysis.
- Hepatitis B
Serum or plasma sample. Minimum volume required for serum or plasma – 1.0 mL.
Collect blood in serum separator tubes (SST) or EDTA tubes.
Store samples frozen until shipped for testing. Ship frozen on dry ice.
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
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Suspected Hepatitis B infection. Serology results must show markers to the Hepatitis B virus and HBV DNA results must be positive (i.e, must have detectable viral load).
Completed Viral Hepatitis and Bloodborne Pathogens requisition including sender name, address and telephone number. Patient name or identifier (referring specimen lab #), date of birth, suspected exposure, test(s) requested. Type of specimen and date collected. If possible, include the clinical history and lab results (particularly HBeAg/eAb test results) that have already been done at local or provincial laboratories.
Conventional PCR followed by 1) line probe hybridization assay, in which reactivity to 1896A (precore) or 1762T/1764A (basal core promoter) probes indicates a mutation, or 2) sequence analysis.
19 calendar days.
- Olivero A, Ciancio A, Abate ML, Gaia S, Smedile A, and Rizzetto M. Performance of sequence analysis, INNO-LiPA line probe assays and AFFIGENE assays in the detection of hepatitis B virus polymerase and precore/core promoter mutations. J Viral Hepatitis 2006 13:355-362.
- Jammeh S, Tavner F, Watson R, et al. Effect of basal core promoter and pre-core mutations on hepatitis B virus replication. J Gen Virol 2008 89:901-909.