Genotyping by Sequence Analysis<<Return to Laboratory
Genotyping of Hepatitis B virus by sequence analysis.
- Hepatitis B
Serum or plasma sample. Minimum volume required for serum or plasma is 1.0 mL.
Collect blood in serum separator tubes (SST) or EDTA tubes.
Store samples frozen until shipped for testing. Ship frozen on dry ice.
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
Suspected Hepatitis B infection. Serology results must show markers to the Hepatitis B virus and HBV DNA results must be positive (i.e., must have detectable viral load).
Completed Viral Hepatitis and Bloodborne Pathogens requisition including sender name, address and telephone number. Patient name or identifier (referring specimen lab #), date of birth, suspected exposure, test(s) requested. Type of specimen and date collected. If possible, include the clinical history and lab results that have already been done at local or provincial laboratories.
Repeated testing of HBV genotype for chronic hepatitis B patients is not warranted unless superinfection is suspected.
Conventional PCR followed by sequencing and phylogenetic analysis in comparison to HBV genotype reference sequences.
19 calendar days.
- McNaughton AL, Revill PA, Littlejohn M, Matthews PC, Ansari MA. Analysis of genomic-length HBV sequences to determine genotype and subgenotype reference sequences. J Gen Virol 2020 101(3):271-283.