Genotyping by PCR

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Requisition Forms

Reference Details

Description:

Genotyping of Hepatitis B virus by sequence analysis.

Test Category:
Genotyping
Pathogen:
Hepatitis B virus (HBV)
Illnesses and Diseases:
  • Hepatitis B
Specimen:

Serum or plasma sample. Minimum volume required for serum or plasma is 1.0 mL.

Collection Method:

Collect blood in serum separator tubes (SST) or EDTA tubes.

Specimen Processing, Storage and Shipping:

Store samples frozen until shipped for testing. Ship frozen on dry ice.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Suspected Hepatitis B infection.  Serology results must show markers to the Hepatitis B virus and HBV DNA results must be positive (i.e, must have detectable viral load).

Accompanying Documentation:

Completed Viral Hepatitis and Bloodborne Pathogens requisition including sender name, address and telephone number.  Patient name or identifier (referring specimen lab #), date of birth, suspected exposure, test(s) requested.  Type of specimen and date collected.  If possible, include the clinical history and lab results that have already been done at local or provincial laboratories.

Comments:

N/A

Methods and Interpretation of Results:

Conventional PCR followed by sequence and phylogenetic analysis in comparison to HBV genotype reference sequences.

Turnaround Time:

19 calendar days.

Contact:
Phone: (204) 789-6061; HCV (204) 789-5063
Fax: (204) 789-2082
References:
  1. Osiowy C, and Giles E. Evaluation of the INNO-LiPA HBV genotyping assay for determination of hepatitis B virus genotype. J Clin Microbiol 2003. 41:5473-5477.
  2. Pourkarim MR, Amini-Bavil-Olyaee S, Kurbanov F, Van Ranst M, and Tacke F. Molecular identification of hepatitis B virus genotypes/subgenotypes: Revised classification hurdles and updated resolutions. World J Gastroenterol 2014. 20:7152-7168.