Genotyping by RT-PCR

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Requisition Forms

Reference Details

Description:

Genotyping of the Hepatitis A virus by conventional RT-PCR followed by DNA sequencing.

Test Category:
Genotyping
Pathogen:
Hepatitis A virus (HAV)
Illnesses and Diseases:
  • Hepatitis A
Specimen:

Serum, plasma or stool sample.  Minimum volume required for serum or plasma is 1.0 mL or approximately 1.0 gram of stool to prepare 20% stool suspension. 

Collection Method:

Collect blood in serum separator tubes (SST) or EDTA tubes. Collect stool sample and freeze.  Optional: prepare 20% stool suspension and freeze immediately.

Specimen Processing, Storage and Shipping:

Store samples frozen until shipped for testing.  Ship frozen on dry ice.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Suspected Hepatitis A infection.  Serology result must be anti-HAV IgM (+).

Accompanying Documentation:

Completed Viral Hepatitis and Bloodborne Pathogens requisition including sender name, address and telephone number.  Patient name or identifier (referring specimen lab #), date of birth, suspected exposure, test(s) requested.  Type of specimen and date collected.  If possible, include the clinical history and lab results that have already been done at local or provincial laboratories.

Comments:

N/A

Methods and Interpretation of Results:

Conventional RT-PCR amplifying the VP1/2A junction followed by sequencing.

Turnaround Time:

19 calendar days.

Contact:
Phone: (204) 789-6061; HCV (204) 789-5063
Fax: (204) 789-2082
References:
  1. Nainan OV, Xia G, Vaughan G, Margolis HS. Diagnosis of hepatitis A virus infection: a molecular approach. Clin Microbiol Rev. 2006 Jan;19(1):63-79.