Genotyping by Sequence Analysis
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Reference Details
Genotyping of the Hepatitis A virus by conventional RT-PCR followed by DNA sequencing.
- Hepatitis A
Serum, plasma or stool sample. Minimum volume required for serum or plasma is 1.0 mL or approximately 1.0 gram of stool to prepare 20% stool suspension.
Collect blood in serum separator tubes (SST) or EDTA tubes. Collect stool sample and freeze. Optional: prepare 20% stool suspension and freeze immediately.
Store samples frozen until shipped for testing. Ship frozen on dry ice.
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
Suspected Hepatitis A infection. Serology result must be anti-HAV IgM (+).
Completed Viral Hepatitis and Bloodborne Pathogens requisition including sender name, address and telephone number. Patient name or identifier (referring specimen lab #), date of birth, suspected exposure, test(s) requested. Type of specimen and date collected. If possible, include the clinical history and lab results that have already been done at local or provincial laboratories.
N/A
Conventional RT-PCR amplifying the VP1/2A junction followed by sequencing and phylogenetic analysis in comparison to HAV genotype reference sequences.
19 calendar days.
- Kroneman A, de Sousa R, Verhoef L, et al., Usability of the international HAVNet hepatitis A virus database for geographical annotation, backtracing and outbreak detection. Euro Surveill. 2018 23(37): 1700802.