Identification and speciation

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*Accredited by the Standards Council of Canada to Laboratory no. 594 - CAN-P-4E (ISO/IEC 17025)

Requisition Forms

Reference Details

Description:

Identification and speciation of Helicobacter spp.

Test Category:
Phenotypic and Molecular Identification
Pathogen:
Helicobacter spp.
Illnesses and Diseases:
  • Peptic ulcer disease
Specimen:

Isolate from urine, stool, blood, environmental, food, other.

 

Collection Method:

Isolate provided on appropriate culture medium with visible growth.

Specimen Processing, Storage and Shipping:

Send as growth on appropriate culture medium or in appropriate transport medium.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Gastroenteritis, septicemia, infection, other.

Accompanying Documentation:

Completed Enteric Diseases Program requisition form.

Comments:

N/A

 

Methods and Interpretation of Results:

Phenotypic Identification (biotyping)*

16S rRNA gene sequencing*

MALDI-TOF*

Helicobacter PCR-RFLP*

  1. Marshall et al., 1999. Rapid Identification of Campylobacter, Arcobacter, and Helicobacter Isolates by PCR-Restriction Fragment Length Polymorphism Analysis of the 16S rRNA Gene. J Clin Micro, 37:4158 – 4160.
  2. Alispahic et al., 2010. Species-Specific Identification and Differentiation of Arcobacter, Helicobacter and Campylobacter by Full-Spectral Matrix-Associated Laser Desorption / Ionization Time of Flight Mass Spectrometry Analysis. J Med Micro, 59:295 - 301.

 

Turnaround Time:

21 calendar days.

Contact:
Phone: (204) 789-6009
Fax: (204) 789-5012
References:
Guidelines: