Identification and speciation

Identification and speciation of Helicobacter spp.

• Peptic ulcer disease

Isolate from urine, stool, blood, environmental, food, other.

Isolate provided on appropriate culture medium with visible growth.

Send as growth on appropriate culture medium or in appropriate transport medium.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Gastroenteritis, septicemia, infection, other.

Completed Enteric Diseases Program requisition form.

N/A

Phenotypic Identification (biotyping)*

16S rRNA gene sequencing*

MALDI-TOF*

Helicobacter PCR-RFLP*

1. Marshall et al., 1999. Rapid Identification of Campylobacter, Arcobacter, and Helicobacter Isolates by PCR-Restriction Fragment Length Polymorphism Analysis of the 16S rRNA Gene. J Clin Micro, 37:4158 – 4160.
2. Alispahic et al., 2010. Species-Specific Identification and Differentiation of Arcobacter, Helicobacter and Campylobacter by Full-Spectral Matrix-Associated Laser Desorption / Ionization Time of Flight Mass Spectrometry Analysis. J Med Micro, 59:295 - 301.

21 calendar days.

Pathogen Safety Data Sheets: Infectious Substances – Helicobacter pylori

National Enteric Surveillance Program (NESP)