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Whole Genome Sequencing (WGS) and Single Nucleotide Polymorphisms (SNPs)

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Accredited by the Standards Council of Canada to Laboratory no. 594 (ISO/IEC 17025)

Requisition Forms

Reference Details


Multi-faceted approach to the molecular typing of Francisella tularensis strains.

WGS Test: Molecular typing of Francisella tularensis strains using Whole Genome Sequencing data.

SNP Test: Molecular typing of Francisella tularensis strains to determine genetic variations between members of a species, using canonical single nucleotide polymorphisms.

Test Category:
Molecular Differentiation
Francisella tularensis
Illnesses and Diseases:
  • Tularemia

Pure bacterial culture.

Collection Method:

Pure bacterial culture – plate or swab.

Specimen Processing, Storage and Shipping:

Store bacterial cultures refrigerated until shipped for testing.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.


Patient Criteria:

Confirmed Francisella tularensis infection.     

Accompanying Documentation:

Completed Bioforensics Assay Development and Diagnostics requisition including sender name, address and telephone number. Patient name or identifier (referring lab #), date of birth, suspected exposure, test(s) requested, clinical history. Type of specimen and date collected. If possible, include the antibiotics administered and travel history. 1



Methods and Interpretation of Results:

WGS Test: The phylogenetic relationships and subtype strains are determined using Whole Genome Sequencing data based on single base pair variants (SNVs) at specific genomic locations.

SNP Test: The SNP pattern will be determined through PCR, looking for single base pair mutations at a specific locus which usually consists of two alleles. 

Turnaround Time:

14 calendar days for a final result.

Phone #: (204) 784-5928 or 1-877-212-6108
Fax: (204) 789-5009
  1. Birdsell DN, et al. 2014 TaqMan Real-Time PCR Assays for Single-Nucleotide Polymorphisms Which Identify Francisella tularensis and ts Subspecies and Subpopulations. PLoS ONE 9(9): e107964.
  2. Keim, P. 2009. Real-Time PCR Canonical SNP assay for Genotyping Francisella tularensis. Northern Arizona University. Guidelines and Standard operating Procedures for Forensic Analysis. V.20Mar09