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Toxin detection

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*Accredited by the Standards Council of Canada to Laboratory no. 594 (ISO/IEC 17025)

Requisition Forms

Reference Details


Toxin detection in Escherichia coli.

Test Category:
Toxin Testing
Escherichia coli
Illnesses and Diseases:
  • Gastroenteritis
  • Septicaemia
  • Bacteraemia
  • Neonatal meningitis

Isolate from urine, stool, blood, environmental, food, other.

Collection Method:

Isolate provided on appropriate culture medium for E. coli.

Specimen Processing, Storage and Shipping:

Send as growth on appropriate culture medium or in appropriate transport medium.  Culture vessel should be leak-proof or sealed appropriately.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.


Patient Criteria:

Gastroenteritis, septicemia, infection, other.

Accompanying Documentation:

Completed Division of Enteric Diseases requisition form.



Methods and Interpretation of Results:

PCR amplification of common toxin genes*(1)

  1. Paton, A.W. and Paton, J. C. 1997. Detection and Characterization of Shiga Toxigenic Escherichia coli by using Multiplex PCR Assays for stx1, stx2, eaeA, Enterohemorrhagic E.coli hlyA, rfb0111, and rfb0157. J. of Clin. Micro. 36: 598 – 602.
Turnaround Time:

7 calendar days for initial toxin type, 15 days for toxin subtyping if required.  Note: Turnaround times for routine isolates may be extended during major foodborne outbreak activities or due to limited availability of resources.

Phone #: (204) 789-6009
Fax: (204) 789-5012