Toxin detection

Toxin detection in Escherichia coli.

• Gastroenteritis
• Septicaemia
• Bacteraemia
• Neonatal meningitis

Isolate from urine, stool, blood, environmental, food, other.

Isolate provided on appropriate culture medium for E. coli.

Send as growth on appropriate culture medium or in appropriate transport medium.  Culture vessel should be leak-proof or sealed appropriately.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Gastroenteritis, septicemia, infection, other.

Completed Division of Enteric Diseases requisition form.

N/A

PCR amplification of common toxin genes*(1)

1. Paton, A.W. and Paton, J. C. 1997. Detection and Characterization of Shiga Toxigenic Escherichia coli by using Multiplex PCR Assays for stx1, stx2, eaeA, Enterohemorrhagic E.coli hlyA, rfb0111, and rfb0157. J. of Clin. Micro. 36: 598 – 602.

7 calendar days for initial toxin type, 15 days for toxin subtyping if required.  Note: Turnaround times for routine isolates may be extended during major foodborne outbreak activities or due to limited availability of resources.

Pathogen Safety Data Sheets: Infectious Substances – Escherichia coli, enterohemorrhagic

Pathogen Safety Data Sheets: Infectious Substances – Escherichia coli, enteroinvasive

Pathogen Safety Data Sheets: Infectious Substances – Escherichia coli, enteropathogenic

Pathogen Safety Data Sheets: Infectious Substances – Escherichia coli, enterotoxigenic

For health professionals: E. coli (Escherichia coli) infection

National case definition: Verotoxigenic Escherichia coli infection

E. coli O-Typer

E. coli (Escherichia coli) infection

National Enteric Surveillance Program (NESP)