Molecular subtyping

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*Accredited by the Standards Council of Canada to Laboratory no. 594 - CAN-P-4E (ISO/IEC 17025)

Requisition Forms

Reference Details

Description:

Molecular subtyping of Escherichia coli.

Test Category:
Molecular Differentiation
Pathogen:
Escherichia coli
Laboratory:
Illnesses and Diseases:
  • Gastroenteritis
Specimen:

Isolate from urine, stool, blood, environmental, food, other.

Collection Method:

Isolate provided on appropriate culture medium for E. coli.

Specimen Processing, Storage and Shipping:

Send as growth on appropriate culture medium or in appropriate transport medium.  Culture vessel should be leak-proof or sealed appropriately.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Gastroenteritis, septicemia, infection, other.

Accompanying Documentation:

Completed Enteric Diseases Program requisition form.

Comments:

N/A

Methods and Interpretation of Results:

Pulsed Field Gel Electrophoresis*

Whole Genome MLST analysis*

Whole Genome SNV analysis

All E. coli O157 will be referred for MLVA testing*

Data will be forwarded to the NML Surveillance, Outbreak Detection and Response section for interpretation according to PulseNet Canada guidelines.  Please contact us using the information below for further information on interpretation guidelines.

Turnaround Time:

 PFGE/MLVA:10 calendar days, Whole genome sequence based methods: 21 calendar days for WGS performed in-house.  5 calendar days for analysis on samples sequenced provincially.  Note: Turnaround times for routine isolates may be extended during major foodborne outbreak activities or due to limited availability of resources.

Contact:
Phone: (204) 789-5067 or (204) 789-7605
Fax: (204) 789-5012
References: