Molecular subtyping<<Return to Laboratory
*Accredited by the Standards Council of Canada to Laboratory no. 594 - CAN-P-4E (ISO/IEC 17025)
Molecular subtyping of Escherichia coli.
Isolate from urine, stool, blood, environmental, food, other.
Isolate provided on appropriate culture medium for E. coli.
Send as growth on appropriate culture medium or in appropriate transport medium. Culture vessel should be leak-proof or sealed appropriately.
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
For additional guidance on the transport of infectious substances in other languages, please click on the link below.
Gastroenteritis, septicemia, infection, other.
Completed Enteric Diseases Program requisition form.
Pulsed Field Gel Electrophoresis*
Whole Genome MLST analysis*
Whole Genome SNV analysis
All E. coli O157 will be referred for MLVA testing*
Data will be forwarded to the NML Surveillance, Outbreak Detection and Response section for interpretation according to PulseNet Canada guidelines. Please contact us using the information below for further information on interpretation guidelines.
PFGE/MLVA:10 calendar days, Whole genome sequence based methods: 21 calendar days for WGS performed in-house. 5 calendar days for analysis on samples sequenced provincially. Note: Turnaround times for routine isolates may be extended during major foodborne outbreak activities or due to limited availability of resources.