Isolation of Ebola virus<<Return to Search Results
Isolation of Ebola virus.
- Ebola virus disease
Whole blood, serum, fresh frozen tissues – Minimum sample 0.5 mL.
Serum: collect in serum (red top) or serum separator tubes (SST), centrifugation of serum tubes is not needed and should be avoided.
Whole blood: collect in EDTA, heparin or citrate tubes, can submit as aliquots in sterile 1.5-2.0 mL screw cap tubes.
Tissues: Fresh frozen tissues should be placed in plastic containers.
Store samples in refrigerator or frozen until being shipped for testing. Serum can be shipped refrigerated or frozen. Avoid freezing whole blood samples. Tissues should be shipped frozen. Ship frozen specimens on dry ice and refrigerated specimens on wet ice.
Store samples in refrigerator or frozen until being shipped for testing. Ship frozen specimens on dry ice and refrigerated samples on wet ice. Shipping of samples suspected of containing Risk Group 4 pathogens shall be done by a TDG certified individual in accordance with TDG regulations. If applicable, initiate an Emergency Response Assistance Plan (ERAP) notification 24 hours prior to shipping. To initiate an ERAP notification, please click the link below to access the transport flow chart for Risk Group 4 pathogens or call 1-866-262-8433 (available 24/7) for assistance.Transport Flowchart for Risk Group 4 Pathogen(s)
Fever (or other symptoms) and travel to filovirus endemic areas or contact to travellers from these areas.
Completed Special Pathogens requisition including sender name, address and telephone number. Patient information including name or identifier, date of birth, and all relevant patient history including clinical diagnosis, underlying diseases, exposure risk and travel history. Indicate type of specimen collected and all specimen information. Select all required tests. If possible, attach lab results that have already been done at local or provincial laboratories.
All patient history must be included; sample without adequate patient history to justify testing are subject to rejection. Contact Special Pathogens prior to submission of sample to ensure appropriate shipping documentation is generated.
For diagnosis of filovirus infection to be confirmed, one or more of the following diagnostic markers must be positive: (i) presence of filovirus-specific IgM or rising titres of IgG, (ii) presence of filovirus RNA by PCR, (iii) isolation of filovirus.
2 calendar days.