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Detection of Antibodies Directed Towards Eastern Equine Encephalitis Virus by PRNT

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Requisition Forms

Reference Details


Serological detection of neutralizing antibodies directed towards Eastern equine encephalitis (EEE) virus by Plaque Reduction Neutralization Test (PRNT).

Test Category:
Eastern Equine Encephalitis virus (EEEV)
Illnesses and Diseases:
  • Eastern Equine Encephalitis

Serum. Minimum volume of 250 µl required.

Collection Method:

2 mL screw cap tubes.

Specimen Processing, Storage and Shipping:

Store samples refrigerated or frozen until shipped for testing. Ship frozen samples on dry ice, and refrigerated samples on wet ice

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.


Patient Criteria:

Suspected EEE infection. Submitted samples will first be analysed by the Viral Zoonoses Diagnostic Laboratory by EEE HAI to determine if the patient has developed anti-EEE antibodies. Samples that are reactive can be further analysed for the presence of neutralizing antibodies. Samples that are HAI non- reactive (negative) will not be tested by PRNT.

Accompanying Documentation:

Completed Viral Zoonoses requisition including sender laboratory name, address and telephone number. Patient name and / or identifier (specimen reference number), date of birth, test(s) requested, collection date of specimen, date of on-set of symptoms, and clinical and travel history of patient.


This is not a routine test. Please contact the Viral Zoonoses Diagnostic Laboratory before sending specimens.

Methods and Interpretation of Results:

The detection of IgG antibodies in a single serum sample is indicative of past or present exposure to this virus. A 4 fold rise or greater in neutralizing antibody titre, or seroconversion in paired sera, is required to document a "confirmed case" of infection.

IgM can persist in serum for up to a year or more after arbovirus exposure.  Thus, detection of IgM by itself is not sufficient for confirmation of acute infection, but is consistent with an exposure at an undetermined time.

Isolation of an arbovirus or detection of nucleic acid by real-time RT-PCR in a clinical specimen provides clear evidence of infection associated with the current clinical illness.


Turnaround Time:

14 Calendar days after the completion of EEE HAI testing.

Phone #: (204) 789-7037
Fax: (204) 789-2018
  1. WHO. 2007. Guidelines for plaque reduction neutralization testing of human antibodies to dengue viruses.
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