Molecular Detection of Eastern Equine Encephalitis (EEE) Virus by Reverse Transcriptase PCR (RT-PCR)<<Return to Search Results
Molecular detection of Eastern equine encephalitis (EEE) virus by reverse transcriptase PCR.
- Eastern Equine Encephalitis
Serum is the preferred specimen; however testing may also be performed on plasma or cerebral spinal fluid (CSF). Other bodily fluids and tissues may be considered for testing, please contact the Viral Zoonoses laboratory to verify suitability. Minimum volume of 600 µl.
Collect blood in serum separator tubes – submit in 2 ml O-ring tube.
Store samples frozen until shipped for testing. Ship frozen on dry ice.
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
Suspected infection with EEE virus.
Completed Viral Zoonoses requisition including sender laboratory name, address and telephone number. Patient name and / or identifier (specimen reference number), date of birth, test(s) requested, collection date of specimen, date of on-set of symptoms, type of specimen, and clinical and travel history of patient.
Please note that the date of sample collection with respect to symptom onset is essential to help determine which tests are conducted and for interpretation of results.
Reverse-transcriptase PCR. Identification of amplicons by direct sequencing. Isolation of an arbovirus, or detection of arboviral antigen or nucleic acid in a clinical specimen would constitute firm evidence of viral association with illness and provide "confirmed case" status.
21 calendar days after date of receipt of specimen.
- Armstrong, P.M., Prince, N., & Andreadis, T.G. (2012). Development of a multi-target TaqMan assay to detect Eastern Equine Encephalitis virus variants in mosquitoes. Vector-Borne and Zoonotic Diseases, 12(N10), 872-876.
- Hull, R., Nattanmai, S., Kramer, L.D., Bernard, K.A., Tavakoli, N.P. (2008). A duplex real-time RT-PCR assay for the detection of St. Louis encephalitis and Eastern equine encephalitis viruses. Diagnostic Microbiology and Infectious Disease, 62(3), 272-279.