Molecular Detection of Dengue Virus by Reverse Transcriptase PCR (RT-PCR)

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Requisition Forms

Reference Details

Description:

Molecular detection of dengue virus by real-time reverse transcriptase PCR. 

Test Category:
Molecular Detection
Pathogen:
Dengue virus
Laboratory:
Illnesses and Diseases:
  • Dengue hemorrhagic fever (DHF)
  • Dengue fever
  • Dengue shock syndrome
Specimen:

Serum is the preferred specimen; however testing may also be performed on plasma or cerebral spinal fluid (CSF).  Other bodily fluids and tissues may be considered for testing, please contact the Viral Zoonoses laboratory to verify suitability. Minimum volume of 600 µl.

Collection Method:

Collect blood in serum separator tubes – submit in 2 ml O-ring tube.  

Specimen Processing, Storage and Shipping:

Store samples frozen until shipped for testing. Ship samples on dry ice.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

 

Patient Criteria:

Suspected dengue virus infection and relevant travel history. 

Accompanying Documentation:

Completed Viral Zoonoses requisition including sender laboratory name, address and telephone number. Patient name and / or identifier (specimen reference number), date of birth, test(s) requested, collection date of specimen, date of on-set of symptoms, type of specimen, and clinical and travel history of patient.

Comments:

Please note that the point of sample collection with respect to symptom onset is essential to help determine which tests are conducted and with interpretation of results.

Methods and Interpretation of Results:

Dengue virus real-time RT-PCR based on TaqMan chemistry. Four separate singleplex assays are performed targeting each of the four dengue serotypes; a result is classified as positive if any of the targets are detected and the Ct value falls within a pre-established cut-off value for that specific assay/serotype. 

Turnaround Time:

21 calendar days.

Contact:
Phone: (204) 789-6097 or (204) 789-2106
Fax: (204) 789-2082
References:
  1. Johnson, B.W., Russell, B.J. and Lanciotti, R.S. Serotype-specific detection of dengue viruses in a fourplex real-time reverse transcriptase PCR assay. 2005. J. Clin. Micro. 43; 4977-4983.
  2. Lanciotti, R.S., Calisher, C.H., Gubler, D.J., Chang, G.J., and Vorndam, V. Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. 1992. J. Clin. Micro. 30; 545-551.
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