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Molecular Detection by Real-Time PCR

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Requisition Forms

Reference Details

Description:

Molecular detection by real-time PCR of Coxiella burnetii.

Test Category:
Molecular Detection
Pathogen:
Coxiella burnetii
Illnesses and Diseases:
  • Q fever
Specimen:

Whole blood – minimum 1.0 mL, biopsy tissue – minimum 1.0 mL.

Collection Method:

Whole blood – EDTA tube; Tissue – submit in a sterile collection tube.

Specimen Processing, Storage and Shipping:

Store blood samples refrigerated until shipped for testing. Store tissue samples frozen until shipped for testing. Ship whole blood at 4oC; ship tissues on dry ice.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Patient Criteria:

Suspected Q fever infection.

Accompanying Documentation:

Completed Rickettsia and Related Zoonotic Diseases requisition including sender name, address and telephone number. Patient name or identifier (referring lab #), date of birth, suspected exposure, test(s) requested, clinical history and symptom onset date. Type of specimen and date collected. If possible, include the antibiotics administered and travel history.

Comments:

N/A

Methods and Interpretation of Results:

Real-time PCR is performed using assays specific for the IS1111 and IS30 insertion sequence elements of C. burnetii.

Turnaround Time:

15 calendar days.

Contact:
Phone #: (204) 789-7037
Fax: (204) 789-2018
References:
  1. Christensen DR, LJ Hartman, BM Loveless, MS Frye, MA Shipley, DL Bridge, MJ Richards, RS Kaplan, J Garrison, CD Baldwin, DA Kulesh DA Norwood. Detection of biological threat agents by real-time PCR: comparison of assay performance on the R.A.P.I.D., the LightCycler, and the Smart Cycler platforms. Clin Chem 2006; 52(1): 141-145.
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