Molecular Detection by Real-Time PCR

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Requisition Forms

Reference Details

Description:

Molecular detection by real-time PCR of Coxiella burnetii.

Test Category:
Molecular Detection
Pathogen:
Coxiella burnetii
Illnesses and Diseases:
  • Q fever
Specimen:

Whole blood – minimum 0.5 mL, biopsy tissue, cerebrospinal fluid (CSF) – minimum 0.5 mL.

Collection Method:

Whole blood – EDTA tube; Tissue – submit in a sterile collection tube.

Specimen Processing, Storage and Shipping:

Store blood samples refrigerated until shipped for testing. Store tissue samples frozen until shipped for testing. Ship whole blood at 4oC; ship tissues on dry ice.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Suspected Q fever infection.

Accompanying Documentation:

Completed Rickettsia and Related Zoonotic Diseases requisition including sender name, address and telephone number. Patient name or identifier (referring lab #), date of birth, suspected exposure, test(s) requested, clinical history vaccination history. Type of specimen and date collected. If possible, include the antibiotics administered and travel history.

Comments:
N/A
Methods and Interpretation of Results:

Real-time PCR is performed using assays specific for the IS1111 and IS30 insertion sequence elements of C. burnetii.

Turnaround Time:

15 calendar days.

Contact:
Phone: (204) 789-7037
Fax: (204) 789-2140
References:
  1. Christensen DR, LJ Hartman, BM Loveless, MS Frye, MA Shipley, DL Bridge, MJ Richards, RS Kaplan, J Garrison, CD Baldwin, DA Kulesh DA Norwood. Detection of biological threat agents by real-time PCR: comparison of assay performance on the R.A.P.I.D., the LightCycler, and the Smart Cycler platforms. Clin Chem 2006; 52(1): 141-145.
Guidelines:
Related Information: