Molecular Detection by Real-Time PCR
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Molecular detection by real-time PCR of Coxiella burnetii.
- Q fever
Whole blood – minimum 1.0 mL, biopsy tissue – minimum 1.0 mL.
Whole blood – EDTA tube; Tissue – submit in a sterile collection tube.
Store blood samples refrigerated until shipped for testing. Store tissue samples frozen until shipped for testing. Ship whole blood at 4oC; ship tissues on dry ice.
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
Suspected Q fever infection.
Completed Rickettsia and Related Zoonotic Diseases requisition including sender name, address and telephone number. Patient name or identifier (referring lab #), date of birth, suspected exposure, test(s) requested, clinical history vaccination history. Type of specimen and date collected. If possible, include the antibiotics administered and travel history.
N/A
Real-time PCR is performed using assays specific for the IS1111 and IS30 insertion sequence elements of C. burnetii.
15 calendar days.
- Christensen DR, LJ Hartman, BM Loveless, MS Frye, MA Shipley, DL Bridge, MJ Richards, RS Kaplan, J Garrison, CD Baldwin, DA Kulesh DA Norwood. Detection of biological threat agents by real-time PCR: comparison of assay performance on the R.A.P.I.D., the LightCycler, and the Smart Cycler platforms. Clin Chem 2006; 52(1): 141-145.