Pulsed Field Gel Electrophoresis (PFGE)

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Requisition Forms

Reference Details

Description:

Pulsed-field gel electrophoresis of Clostridium difficile.

Test Category:
Genetic Fingerprinting
Pathogen:
Clostridium difficile
Illnesses and Diseases:
  • Pseudomembranous colitis
Specimen:

Pure culture or 1.0mL frozen stool sample.

Collection Method:

N/A

Specimen Processing, Storage and Shipping:

Send cultures as growth on culture medium or in transport medium.  Send frozen stool samples on dry ice.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

N/A

Accompanying Documentation:

Completed Antimicrobial Resistance and Nosocomial Infections Requisition.

Comments:

PFGE will only be applied for outbreak investigations or comparative purpose. Please consult lab prior to requesting test.

Methods and Interpretation of Results:

Fingerprints are compared to a national database.

Turnaround Time:

10 calendar days.

Contact:
Phone: (204) 789-5000
Fax: (204) 789-5020
References:
  1. Miller, M. et al. 2010. Health Care-Associated Clostridium difficile Infection in Canada: Patient Age and Infecting Strain Type Are Highly Predictive of Severe Outcome and Mortality. Clinical Infectious Diseases, vol. 50(2), pg. 194-201
  2. Alfa M. J., Kabani A., Lyerly D., et al. 2000. Characterization of a toxin A-negative, toxin B-positive strain of Clostridium difficile responsible for a nosocomial outbreak of Clostridium difficile-associated diarrhea. Journal of Clinical Microbiology.  38(7):2706–2714.