Molecular Detection and Genotyping by Sequencing

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Accredited by the Standards Council of Canada to Laboratory no. 594 (ISO/IEC 17025)

Requisition Forms

Reference Details

Description:

Detection of LGV Chlamydia serovars by real-time PCR on confirmed C. trachomatis samples and genotyping by direct sequencing of confirmed LGV serovars.

Test Category:
Molecular Detection and Genotyping
Pathogen:
Chlamydia trachomatis - Lymphogranuloma venereum (LGV)
Illnesses and Diseases:
  • Lymphogranuloma venereum (LGV)
Specimen:

Chlamydia positive culture or positive rectal, endocervical, urethral, urine,  biopsies as well as specimens in Roche Cobas 4800 CTNG buffer and specimens in GEN-PROBE APTIMA buffer.  The minimum volume required is 1mL.

Collection Method:

Swab or cytobrush

Specimen Processing, Storage and Shipping:

Place cytobrushes or swabs in 2 mL of transport medium. Specimens may be stored and shipped refrigerated to NML within 48h of sample collection or must be stored and shipped frozen. Fresh biopsies must be stored frozen at all times and shipped on dry ice.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

 

Patient Criteria:

This service is offered for LGV suspected cases on positive C. trachomatis samples only. Specimens for C. trachomatis screening are not accepted unless part of pre-arranged collaborative study.

Accompanying Documentation:

Completed “Viral STI, Polyoma and Herpesvirus Testing” requisition form including sender lab name, address and telephone number, patient identifier, date of birth or age, sex, specimen reference #, type of specimen, date collected, test requested, reason for testing and any other relevant clinical information.

Please contact the VESTD lab if you wish to use an excel sheet format requisition to accommodate batch submission of specimens.

Comments:

N/A

Methods and Interpretation of Results:

Detection of LGV Chlamydia in Chlamydia positive specimens is performed by a multiplex real time PCR assay (1). The analytical sensitivity for the detection of LGV is between 10 and 100 copies/reaction while the detection for the cryptic plasmid is about 100 copies/reaction.  The serovar is confirmed by amplification and sequencing of the ompA gene and the pmpH gene. 

Please note that this method is less sensitive than diagnostic kits available and is not intended for Chlamydia trachomatis screening. 

Turnaround Time:

10 calendar days for LGV detection and 21 calendar days for LGV genotyping.  Please contact lab to inquire about shorter turn around time.

Contact:
Phone: (204) 789-6024
Fax: (204) 318-2222
References:
  1. Chen CY, Chi KH, Alexander S, Ison CA, Ballard RC. A real-time quadriplex PCR assay for the diagnosis of rectal lymphogranuloma venereum and non-lymphogranuloma venereum Chlamydia trachomatis infections. Sex Transm Infect. 2008 Aug;84(4):273-6.