Identification
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Requisition Forms
Reference Details
Identification of Campylobacter spp.
- Campylobacteriosis
Isolate from urine, stool, blood, environmental, food, other.
Isolate provided on appropriate culture medium with visible growth.
Send as growth on appropriate culture medium or in appropriate transport medium.
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
Gastroenteritis, septicemia, infection, other.
Completed Enteric Diseases Program requisition form.
N/A
Phenotypic Identification (biotyping)*(1)
16S rRNA gene sequencing*(2)
MALDI-TOF*(3)
Species-specific PCR based identification (C. jejuni*, C. coli*, C. fetus*) (4,5)
Whole genome sequencing based methodologies may be applied
- Minimal standards for describing new species belonging to the families Campylobacteraceae and Helicobacteraceae: Campylobacter, Arcobacter, Helicobacter and Wolinella spp. On, et. al., 2017. Int J Syst Evol Microbiol. Dec;67(12):5296-5311.
- Sequences compared to the 16s rDNA database in NCBI and clustered using BioNumerics
- MALDI-TOF results analysed using the most current Bruker Biotyper database.
- PCR detection, identification to species level, and fingerprinting of Campylobacter jejuni and Campylobacter coli direct from diarrheic samples. Linton et. al. 1997. J Clin Microbiol. Oct; 35(10): 2568–2572
- Evaluation of a PCR Assay for Identification and Differentiation of Campylobacter fetus subspecies. Hum et. al. 1997. Aust Vet J. 75:827-831
Please contact the laboratory using the information provided below for further information on specific interpretation guidelines.
21 calendar days.