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Multi-Locus Sequence Typing (MLST)

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Requisition Forms

Reference Details

Description:

Multi-locus sequence typing (MLST) of Burkholderia cepacia complex (Bcc) for speciation and typing purposes.

Test Category:
Genetic Fingerprinting
Pathogen:
Burkholderia cepacia complex
Illnesses and Diseases:
  • Pneumonia
Specimen:

Pure culture.

Collection Method:

Slants or plates of any suitable media and swabs in transport media are all acceptable.

Specimen Processing, Storage and Shipping:

Store samples between room temperature and 37oC until shipped for testing. Ship at room temperature.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Patient Criteria:

Respiratory infection by Burkholderia cepacia complex or other infections in immunocompromised patients

Accompanying Documentation:

Completed Special Bacteriology requisition form detailing all patient information and relevant clinical information. If possible, attach lab results that have already been done at local or provincial laboratories.

 

Comments:

All patient and strain history must be included. 

Methods and Interpretation of Results:

Genome sequence derived MLST for Bcc for the purpose of speciation within the Bcc and for strain typing.  Sequences are compared against an on-line database.

Turnaround Time:

21 calendar days.  In cases where staff or resources are limited, or where a novel allele needs number assignment from the PubMLST curator, the final report may be delayed and status of request will be forwarded in a preliminary report.

Contact:
Phone #: (204) 789-2137
Fax: (204) 784-7509
References:
  1. Baldwin et al.  2005.  Multilocus Sequence Typing Scheme That Provides Both Species and Strain Differentiation for the Burkholderia cepacia Complex.  JCM 43(9): 4665-4673.
  2. Burkholderia cepacia complex Multi Locus Sequence Typing website (http://pubmlst.org/bcc/) developed by Keith Jolley and cited as Jolley KA, Bray JE, Maiden MCJ. Open-access bacterial population genomics: BIGSdb software, the PubMLST.org website and their applications. Wellcome Open Res. 2018 Sep 24;3:124. doi: 10.12688/wellcomeopenres.14826.1
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