Multi-Locus Sequence Typing (MLST)

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Requisition Forms

Reference Details

Description:

Multi-locus sequence typing (MLST) of Burkholderia cepacia complex (Bcc) for speciation and typing purposes.

Test Category:
Genetic Fingerprinting
Pathogen:
Burkholderia cepacia complex
Illnesses and Diseases:
  • Pneumonia
Specimen:

Pure culture.

Collection Method:

Slants or plates of any suitable media and swabs in transport media are all acceptable.

Specimen Processing, Storage and Shipping:

Store samples between room temperature and 37oC until shipped for testing. Ship at room temperature.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Respiratory infection by Burkholderia cepacia complex.

Accompanying Documentation:

Completed Special Bacteriology requisition form detailing all patient information and relevant clinical information. If possible, attach lab results that have already been done at local or provincial laboratories.

 

Comments:

All patient and strain history must be included.

Methods and Interpretation of Results:

MLST for Bcc involves the amplification and sequencing of seven target genes for the purpose of speciation within the Bcc and for strain typing. Sequences are compared against an on-line database.

Turnaround Time:

30 calendar days.  In cases where staff or resources are limited, or where  a novel allele needs number assignment from the PUBMLST curator, the final report may be delayed and status of request will be forwarded in a preliminary report.

Contact:
Phone: (204) 789-2137
Fax: (204) 784-7509
References:
  1. Baldwin et al. 2005. Multilocus Sequence Typing Scheme That Provides Both Species and Strain Differentiation for the Burkholderia cepacia Complex. JCM 43(9): 4665-4673.
  2. Burkholderia cepacia complex Multi Locus Sequence Typing website (http://pubmlst.org/bcc/) developed by Keith Jolley and sited at the University of Oxford ( Jolley et al. 2004, BMC Bioinformatics, 5:86).
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