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Multi-Locus Sequence Typing (MLST)

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Reference Details


Multi-locus sequence typing (MLST) of Burkholderia cepacia complex (Bcc) for speciation and typing purposes.

Test Category:
Molecular Typing
Burkholderia cepacia complex
Illnesses and Diseases:
  • Pneumonia

Pure culture.

Collection Method:

Slants (preferred) or plates of any suitable media and swabs in transport media are all acceptable. Frozen specimens are also acceptable however, may extend turnaround time. 

Specimen Processing, Storage and Shipping:

Store slants and swabs at room temperature until shipped for testing. Ship actively growing cultures at room temperature. Frozen specimens should be kept frozen and shipped on dry ice.


Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Patient Criteria:

Respiratory infection by Burkholderia cepacia complex or other infections in immunocompromised patients

Accompanying Documentation:

Completed Special Bacteriology requisition form (most recent version of requisition is required), detailing all patient information and relevant clinical information.  If possible, attach lab results that have already been done at local or provincial laboratories.


All patient and strain history must be included. Indicate if patient has cystic fibrosis.



Methods and Interpretation of Results:

Genome sequence derived MLST for Bcc for the purpose of speciation within the Bcc and for strain typing.  Sequences are compared against an on-line database.

Turnaround Time:

21 calendar days.  In cases where staff or resources are limited, or where a novel allele needs number assignment from the PubMLST curator, the final report may be delayed and status of request will be forwarded in a preliminary report.

Phone #: (204) 789-2137
Fax: (204) 784-7509
  1. Baldwin et al.  2005.  Multilocus Sequence Typing Scheme That Provides Both Species and Strain Differentiation for the Burkholderia cepacia Complex.  JCM 43(9): 4665-4673.
  2. Burkholderia cepacia complex Multi Locus Sequence Typing website ( developed by Keith Jolley and cited as Jolley KA, Bray JE, Maiden MCJ. Open-access bacterial population genomics: BIGSdb software, the website and their applications. Wellcome Open Res. 2018 Sep 24;3:124. doi: 10.12688/wellcomeopenres.14826.1
  3. Jin et al. Genome-based classification of Burkholderia cepacia complex provides new insight into its taxonomic status. Biology Direct (2020) 15:6
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