Immunoblot (Western Blot) – IgG

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Accredited by the Standards Council of Canada to Laboratory no. 594 - CAN-P-4E (ISO/IEC 17025)

Requisition Forms

Reference Details


Detection of IgG antibodies to whole antigens plus recombinant VlsE of Borrelia burgdorferi sensu stricto (North American strain) by Western blot assay.

Test Category:
Borrelia burgdorferi
Illnesses and Diseases:
  • Lyme Disease

Fresh human serum.  Minimum volume of serum required is 0.25 mL.  

Collection Method:

Collect blood in serum separator tubes.

Specimen Processing, Storage and Shipping:

Transfer an aliquot of serum to a 1.5 ml screw-top vial with O-ring (Sarstedt vial). Store specimens refrigerated up to 5 days or store frozen until shipped for testing. Ship frozen on dry or with freezer packs.  

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.


Patient Criteria:

Appropriate clinical symptoms with potential exposure to blacklegged ticks. 

The IgG Western blot is intended for use on human serum samples which have been found positive or equivocal by the ELISA procedure to provide supportive evidence of infection with B. burgdorferi.

Accompanying Documentation:

Completed Requisition for Serological Testing for Selected Zoonotic Agents. If possible, include the clinical history and lab results performed at local or provincial laboratories.


Specimens may be subject to rejection if they are not the appropriate sample type, have insufficient volume, or are not accompanied by relevant patient information.

Methods and Interpretation of Results:

Commercial Western blot assay. This is a qualitative test for the detection of IgG antibodies to individual proteins of B. burgdorferi sensu stricto. If early Borreliosis is suspected, serologic evidence of infection is best obtained by testing paired acute and convalescent phase serum samples collected 3 – 6 weeks apart.

Initiation of antibiotic treatment prior to testing may result in decreased antibody production which will affect the outcome of serological testing. However, if Lyme disease is suspected based on clinical symptoms, treatment should be initiated.

Turnaround Time:

21 calendar days.

Phone: (204) 789-7434 or (204) 789-7662
Fax: (204) 789-2082
  1. Moore A, Nelson C, Molins C, Mead P, & Schriefer M. 2016. Current Guidelines, Common Clinical Pitfalls, and Future Directions for Laboratory Diagnosis of Lyme Disease, United States. Emerging Infectious Diseases 22(7): 1169-1177.
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