Enzyme-linked Immunosorbent Assay (ELISA) on Serum<<Return to Laboratory
Accredited by the Standards Council of Canada to Laboratory no. 594 (ISO/IEC 17025)
Detection of IgG/IgM antibodies to VlsE1 and pepC10 antigens derived from Borrelia burgdorferi sensu lato by enzyme-linked immunosorbent assay (ELISA).
- Lyme Disease
Fresh human serum.
Minimum volume required is 0.25 mL.
Hemolytic or lipemic sera may yield anomalous results.
Collect blood in serum separator tubes.
Transfer an aliquot of serum to a 1.5 ml screw-top vial with O-ring (Sarstedt vial). Store specimens refrigerated up to 5 days or store frozen until shipped for testing. Ship frozen on dry ice or with freezer packs.
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
Appropriate clinical symptoms with potential exposure to blacklegged ticks. Typical symptoms include fever, headache, fatigue, and a characteristic rash may be present. If left untreated, infection can spread to joints, the heart, and the nervous system.
Completed Requisition for Serological Testing for Selected Zoonotic Agents. If possible, include the clinical history and lab results performed at local or provincial laboratories.
Specimens may be subject to rejection if they are not the appropriate sample type, have insufficient volume, or are not accompanied by relevant patient information.
This commercial ELISA is a qualitative test for the detection of IgG and/or IgM antibodies to B. burgdorferi sensu stricto and pathogenic European genospecies of Borrelia.
Samples that are reactive or equivocal by this ELISA are subject to confirmation by immunoblot.
If the sample is negative by the ELISA and/or immunoblot, a follow-up specimen should be submitted after an additional 3 to 6 weeks.
Initiation of antibiotic treatment prior to testing may result in decreased antibody production which will affect the outcome of serological testing. However, if Lyme disease is suspected based on clinical symptoms, treatment should be initiated.
21 calendar days.
Moore A, Nelson C, Molins C, Mead P, & Schriefer M. 2016. Current Guidelines, Common Clinical Pitfalls, and Future Directions for Laboratory Diagnosis of Lyme Disease, United States. Emerging Infectious Diseases 22(7): 1169-1177.