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Requisition Forms

Reference Details


Sera analyzed for IgG response against Bacillus anthracis protective antigen via ELISA and possibly via B. anthracis lethal toxin neutralization activity.

Test Category:
Bacillus anthracis
Illnesses and Diseases:
  • Anthrax

Serum sample. Minimum volume required – 1.0 – 2.0 mL. Preferred sample at least 10 days from the onset of symptoms. Also, recommend obtaining another sample 14 days after previous sample tested.

Collection Method:


Specimen Processing, Storage and Shipping:

Store samples refrigerated or frozen until shipped for testing. Ship frozen samples on dry ice, and refrigerated samples on wet ice

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.


Patient Criteria:

Suspected exposure to B. anthracis. Special request only. Please contact the laboratory prior to submission of specimen.

Accompanying Documentation:

Please list all clinical symptoms appropriate to the suspected area of infection1 detailed by submission of a completed Bioforensics Assay Development and Diagnostics requisition form and clearly specify organism testing request on the requisition form. Append all relevant clinical background information and testing performed.1


Special request only.  Not recommended if patient is on post-exposure prophylaxis. Please contact the laboratory prior to submission of specimen. Please note this is not an accredited test.

Methods and Interpretation of Results:

ELISA conducted under similar conditions as in reference 2.

Turnaround Time:

14 calendar days. Please note that during times when large numbers of samples are received or if tests must be repeated the turnaround time may be longer.

Phone: (204) 784-5928 or 1-877-212-6108
Fax: (204) 789-5009
  1. Semenova, V.A. Steward-Clark, E. Stamey, K.L., Taylor, T.H., Schmidt, D.S., Martin S.K., Marano and C.P. Quinn. 2004. Clin. Diagnos. Lab. Immu. 11 (5); 919-923.
  2. Quinn, C.P. et al. 2004. Journal of Infectious Diseases. 190: 1228-36.
  3. Gubbins, M.J., Berry, J.D., Corbett, C.R., Mogridge, J, Yuan, X., Schmidt, L., Nicolas, B., Dabani, A. and R. Tsang. 2006. FEMS Immun Med Microbiol. (47); 436-443.
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