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Requisition Forms

Reference Details


Sera analyzed for IgG response against Bacillus anthracis protective antigen via ELISA.

Test Category:
Bacillus anthracis
Illnesses and Diseases:
  • Anthrax

Serum sample. Minimum volume required – 1.0 – 2.0 mL. Preferred sample at least 10 days from the onset of symptoms. Also, recommend obtaining another sample 14 days after previous sample tested.

Collection Method:

Collect blood in serum separator tubes.

Specimen Processing, Storage and Shipping:

Store specimens refrigerated up to 5 days or frozen until shipped for testing. Ship frozen on dry ice or on wet ice. 

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.


Patient Criteria:

Suspected exposure to B. anthracis. Special request only. Please contact the laboratory prior to submission of specimen.

Accompanying Documentation:

Please list all clinical symptoms appropriate to the suspected area of infection detailed by submission of a completed Bioforensics Assay Development and Diagnostics requisition form including sender name, address and telephone number, patient name or identifier (referring lab #), date of birth, suspected exposure, test(s) requested, type of specimen and date collected. Append all relevant clinical background information and testing performed. Please include patient travel history if applicable.


Special request only.  Not recommended if patient is on post-exposure prophylaxis. Please contact the laboratory prior to submission of specimen. Please note this is not an accredited test.

Methods and Interpretation of Results:

ELISA conducted under similar conditions as in reference 2.

Turnaround Time:

14 calendar days. Please note that during times when large numbers of samples are received or if tests must be repeated the turnaround time may be longer.

Phone #: (204) 784-5928 or 1-877-212-6108
Fax: (204) 789-5009
  1. Semenova, VA et al. 2004. Mass value assignment of total and subclass immunoglobulin G in a human standard anthrax reference serum. Clin. Diagnos. Lab. Immu. 11 (5); 919-923.
  2. Quinn, CP et al. 2004. Immune responses to Bacillus anthracis protective antigen in patients with bioterrorism-related cutaneous or inhalation anthrax. J Infect Dis. 190: 1228-36.
  3. Gubbins, ML, et al. 2006. Production and characterization of neutralizing monoclonal antibodies that recognize an epitope in domain 2 of Bacillus anthracis protective antigen. FEMS Immun Med Microbiol. (47); 436-443.
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