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Requisition Forms

Reference Details

Description:

Sera analyzed for IgG response against Bacillus anthracis protective antigen via ELISA.

Test Category:
Serology
Pathogen:
Bacillus anthracis
Illnesses and Diseases:
  • Anthrax
Specimen:

Serum sample. Minimum volume required – 1.0 – 2.0 mL. Preferred sample at least 10 days from the onset of symptoms. Also, recommend obtaining another sample 14 days after previous sample tested.

Collection Method:

Collect blood in serum separator tubes.

Specimen Processing, Storage and Shipping:

Store specimens refrigerated up to 5 days or frozen until shipped for testing. Ship frozen on dry ice or on wet ice. 

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

 

Patient Criteria:

Suspected exposure to B. anthracis. Special request only. Please contact the laboratory prior to submission of specimen.

Accompanying Documentation:

Please list all clinical symptoms appropriate to the suspected area of infection detailed by submission of a completed Bioforensics Assay Development and Diagnostics requisition form including sender name, address and telephone number, patient name or identifier (referring lab #), date of birth, suspected exposure, test(s) requested, type of specimen and date collected. Append all relevant clinical background information and testing performed. Please include patient travel history if applicable.

Comments:

Special request only.  Not recommended if patient is on post-exposure prophylaxis. Please contact the laboratory prior to submission of specimen. Please note this is not an accredited test.

Methods and Interpretation of Results:

ELISA conducted under similar conditions as in reference 2.

Turnaround Time:

14 calendar days. Please note that during times when large numbers of samples are received or if tests must be repeated the turnaround time may be longer.

Contact:
Phone #: (204) 784-5928 or 1-877-212-6108
Fax: (204) 789-5009
References:
  1. Semenova, VA et al. 2004. Mass value assignment of total and subclass immunoglobulin G in a human standard anthrax reference serum. Clin. Diagnos. Lab. Immu. 11 (5); 919-923.
  2. Quinn, CP et al. 2004. Immune responses to Bacillus anthracis protective antigen in patients with bioterrorism-related cutaneous or inhalation anthrax. J Infect Dis. 190: 1228-36.
  3. Gubbins, ML, et al. 2006. Production and characterization of neutralizing monoclonal antibodies that recognize an epitope in domain 2 of Bacillus anthracis protective antigen. FEMS Immun Med Microbiol. (47); 436-443.
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