Whole Genome Sequencing (WGS) and Single Nucleotide Polymorphisms (SNPs)
<<Return to LaboratoryAccredited by the Standards Council of Canada to Laboratory no. 594 (ISO/IEC 17025)
Requisition Forms
Reference Details
Multi-faceted approach to the molecular typing of Bacillus anthracis strains.
WGS Test: Molecular typing of Bacillus anthracis strains using Whole Genome Sequencing data.
SNP Test: Molecular typing Bacillus anthracis strains to determine genetic variations between members of a species, using canonical single nucleotide polymorphisms.
- Anthrax
Pure bacterial culture
Pure bacterial culture – plate or swab
Store bacterial cultures refrigerated until shipped for testing.
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
Confirmed Bacillus anthracis infection.
Completed Bioforensics Assay Development and Diagnostics requisition including sender name, address and telephone number. Patient name or identifier (referring lab #), date of birth, suspected exposure, test(s) requested, clinical history. Type of specimen and date collected. If possible, include the antibiotics administered and travel history.
N/A
WGS Test: The phylogenetic relationships and subtype strains are determined using Whole Genome Sequencing data based on single base pair variants (SNVs) at specific genomic locations.
SNP Test: The SNP pattern will be determined through PCR, looking for single base pair mutations at a specific locus which usually consists of two alleles.
14 calendar days for a final result.
- Keim, P. 2008 Real-Time PCR, plcR and Canonical SNP Genotyping of Bacillus anthracis.Northern Arizona University. Guidelines and Standard operating Procedures for Forensic Analysis. V04.29.08
- Van Ert MN, et al. 2007. Global Genetic Population Structure of Bacillus anthracis. PLoS ONE. 2(5): e461.
- Tyler AD, et al. 2018. Evaluation of Oxford Nanopore's MinION Sequencing Device for Microbial Whole Genome Sequencing Applications. Sci Rep. Jul 19;8(1):10931.