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Whole Genome Sequencing (WGS) and Single Nucleotide Polymorphisms (SNPs)

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Accredited by the Standards Council of Canada to Laboratory no. 594 (ISO/IEC 17025)

Requisition Forms

Reference Details


Multi-faceted approach to the molecular typing of Bacillus anthracis strains.

WGS Test: Molecular typing of Bacillus anthracis strains using Whole Genome Sequencing data.

SNP Test: Molecular typing Bacillus anthracis strains to determine genetic variations between members of a species, using canonical single nucleotide polymorphisms.

Test Category:
Molecular Differentiation
Bacillus anthracis
Illnesses and Diseases:
  • Anthrax

Pure bacterial culture

Collection Method:

Pure bacterial culture – plate or swab

Specimen Processing, Storage and Shipping:

Store bacterial cultures refrigerated until shipped for testing.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.


Patient Criteria:

Confirmed Bacillus anthracis infection. 

Accompanying Documentation:

Completed Bioforensics Assay Development and Diagnostics requisition including sender name, address and telephone number. Patient name or identifier (referring lab #), date of birth, suspected exposure, test(s) requested, clinical history. Type of specimen and date collected. If possible, include the antibiotics administered and travel history.



Methods and Interpretation of Results:

WGS Test: The phylogenetic relationships and subtype strains are determined using Whole Genome Sequencing data based on single base pair variants (SNVs) at specific genomic locations.

SNP Test: The SNP pattern will be determined through PCR, looking for single base pair mutations at a specific locus which usually consists of two alleles. 

Turnaround Time:

14 calendar days for a final result.

Phone #: (204) 784-5928 or 1-877-212-6108
Fax: (204) 789-5009
  1. Keim, P. 2008 Real-Time PCR, plcR and Canonical SNP Genotyping of Bacillus anthracis.Northern Arizona University. Guidelines and Standard operating Procedures for Forensic Analysis. V04.29.08
  2. Van Ert MN, et al. 2007. Global Genetic Population Structure of Bacillus anthracis. PLoS ONE. 2(5): e461.
  3. Tyler AD, et al. 2018. Evaluation of Oxford Nanopore's MinION Sequencing Device for Microbial Whole Genome Sequencing Applications. Sci Rep. Jul 19;8(1):10931.
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