Molecular Detection of Bacillus anthracis

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Accredited by the Standards Council of Canada to Laboratory no. 594 - CAN-P-4E (ISO/IEC 17025)

Requisition Forms

Reference Details

Description:

Molecular detection by real-time PCR of Bacillus anthracis.

Test Category:
Molecular Detection
Pathogen:
Bacillus anthracis
Illnesses and Diseases:
  • Anthrax
Specimen:

Pure bacterial culture, whole blood – minimum 0.5 mL, cerebrospinal fluid (CSF) – minimum 0.5 mL, biopsy tissue, powder.

Collection Method:

Pure bacterial culture – plate or swab; Whole blood – EDTA tube; Tissue or CSF – submit in a sterile collection tube; powder – submit in a sterile 2 mL o-ring microcentrifuge tube.

Specimen Processing, Storage and Shipping:

Store blood samples refrigerated until shipped for testing. Store tissue samples and CSF frozen until shipped for testing. Store bacterial cultures refrigerated until shipped for testing.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Suspected Bacillus anthracis infection.

Accompanying Documentation:

Completed Bioforensics Assay Development and Diagnostics requisition including sender name, address and telephone number. Patient name or identifier (referring lab #), date of birth, suspected exposure, test(s) requested, clinical history. Type of specimen and date collected. If possible, include the antibiotics administered and travel history.

Comments:

N/A

Methods and Interpretation of Results:

Real-time PCR is performed using in-house differentiating molecular assays specific for the pXO1 and pXO2 regions of Bacillus anthracis, as well as a Bacillus anthracis chromosomal target.

Confirmatory tests of a sample positive for Bacillus anthracis by real-time PCR result may include gamma phage lysis, motility, penicillin sensitivity, Single Nucleotide Polymorphisms (SNPs), Multiple Locus Variable Number Tandem Repeat Analysis (MLVA), and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) bacterial identification.

MALDI-TOF MS will be performed on all samples negative for Bacillus anthracis by real-time PCR, as long as the sample provides bacterial growth by culture.

Turnaround Time:

4 calendar days for a preliminary result; 14 calendar days for a final result; 28 days for any non-standard specimen type or specialized testing requests.

Contact:
Phone: (204) 784-5928 or 1-877-212-6108
Fax: (204) 789-5009
References:
  1. Tracz, D.M., McCorrister, S.J., Westmacott, G.R., Corbett, C.R., 2012.  Effect of gamma radiation on the identification of bacterial pathogens by MALDI-TOF MS. J. Microbiol. Methods 92, 132-134.
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