Identification

Identification of Aeromonas spp.

• Gastroenteritis

Isolate from urine, stool, blood, environmental, food, other.

Isolate provided on appropriate culture medium with visible growth.

Send as growth on appropriate culture medium or in appropriate transport medium.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Gastroenteritis, septicemia, infection, other.

Completed Division of Enteric Diseases requisition form.

N/A

Phenotypic Identification (biotyping)*(1)

16S rRNA gene sequencing(2)

MALDI-TOF(3)

Whole genome sequencing based methodologies may be applied

1. Biotyping: as per Murray, P.R., Baron, E.J., Jorgensen, J.H., Pfaller, M.A., Yolken, R.H., Manual of Clinical Microbiology. 2003. ASM Press. Washington, DC. USA
2. Sequences compared to the 16s rDNA database in NCBI and clustered using BioNumerics
3. MALDI-TOF results analysed using the most current Bruker Biotyper database.

21 calendar days.

Pathogen Safety Data Sheets: Infectious Substances – Aeromonas hydrophila

National Enteric Surveillance Program (NESP)