Genotyping by PCR and Sequencing

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Requisition Forms

Reference Details

Description:

Genotyping of Adenovirus spp. by PCR and sequencing.

Test Category:
Genotyping
Pathogen:
Adenovirus
Illnesses and Diseases:
  • Respiratory tract infections
Specimen:

Viral isolates (1.0 – 2.0 mL). 

Collection Method:

N/A

Specimen Processing, Storage and Shipping:

Keep samples refrigerated until shipped for testing. Ship on dry ice.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Confirmed Adenovirus infection.

Accompanying Documentation:

Completed requisition (current Adenovirus Genotyping requisition form).  Sender lab name, address, and telephone number must be provided for samples to be processed.  Also include identifier (sender lab number), patient date of birth, patient gender, and date collected.  Under additional information, please include if possible/applicable patient travel history and date of collection relative to antiviral use.

Comments:

Authorization must be obtained for genotyping by PCR and sequencing for Adenovirus spp.  Please contact the Influenza and Respiratory Viruses laboratory before sending specimens.

Methods and Interpretation of Results:

Nucleic acids are extracted from submitted isolates.  Polymerase Chain Reaction (PCR) with Adenovirus-specific primers is performed on extracted nucleic acids.  If initial PCR is unsuccessful, nested PCR with additional Adenovirus-specific primers is performed.    PCR products are sequenced and aligned with reference sequences to determine the genotype.

Turnaround Time:

14 calendar days.

Contact:
Phone: (204) 789-6045
Fax: (204) 789-2082
References:
  1. X Lu, DD Erdman. Molecular typing of human adenoviruses by PCR and sequencing of a partial region of the hexon gene. Arch Virol (2006) 151: 1587-1602.
Guidelines:
Related Information: