Molecular Detection by Conventional PCR and Sequencing

Detection of Trichodysplasia Spinulosa Polyomavirus DNA in tissue specimens.

Fresh biopsy or fixed tissue. For fixed tissue, please submit 5 scrolls or entire block.

Fresh biopsy or fixed tissue.

Fresh biopsies must be stored frozen at all times and shipped on dry ice. Fixed tissue may be stored and shipped at room temperature.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Suspected Trichodysplasia spinulosa presenting with characteristic folliculocentric lesions of the central face in immunocompromised patients (1). Primarily identified in lesional skin, TSPyV may also be detected in other tissues in the context of systemic involvement.

Completed “Sexually Transmitted and Opportunistic Infections” requisition form including sender lab name, address and telephone number, patient identifier, date of birth or age and sex, specimen reference #, type of specimen, date collected, test requested, and any other relevant clinical information.

SPECIAL REQUEST ONLY. Contact the laboratory prior to sending specimens.

Conventional PCR with TSPyV specific primers targeting the small T antigen viral gene followed by direct sequencing (1). The sequence data are then analyzed and compared to published Trichodysplasia Spinulosa Polyomavirus sequences. Results are for research purposes only.

Testing is performed, in whole or in part, using a “Research Use Only” testing kit which has not been fully validated/verified.

Test is done by special request; inquire with lab for turnaround times.

1. Fischer MK, Kao GF, Nguyen HP, et al. Specific Detection of Trichodysplasia Spinulosa–Associated Polyomavirus DNA in Skin and Renal Allograft Tissues in a Patient With Trichodysplasia Spinulosa. Arch Dermatol. 2012;148(6):726–733. doi:10.1001/archdermatol.2011.3298