Identification

Phenotypic Identification and Serotyping of Vibrio cholerae

Isolate (pure culture) from urine, stool, blood, environmental, food, other.

Isolate provided on solid culture medium for Vibrio cholerae (slant/stab).

Send as growth on solid culture medium or in appropriate solid transport medium. Culture vessel should be leak-proof or sealed appropriately.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Gastroenteritis, septicemia, infection, other.

Completed Enteric Diseases requisition form.

N/A

Phenotypic Identification: Comparison of observed (phenotypic) reactions from a panel of substrate utilization tests against published expected reactions.

Serotyping: Slide agglutination assay for identification of outbreak-associated serotypes: O1, O139, O75 and O141.

Whole genome sequencing based methodologies may be applied to aid in species confirmation (Not accredited to ISO/IEC 17025 at this time).

7 calendar days. Note: Turnaround times for routine isolates may be extended during major foodborne outbreak activities or due to limited availability of resources.

1. Murray, P.R., Baron, E.J., Jorgensen, J.H., Pfaller, M.A., Yolken, R.H., Manual of Clinical Microbiology. 2003. ASM Press. Washington, DC. USA
2. Laboratory Methods for the Diagnosis of Cholera Vibrio and Other Vibrios. 1991. Institut Pasteur 28, rue du Docteur-Roux 75724 PARIS CEDEX 15 - FRANCE.