Dried Blood Spot Testing

HIV – Dried Blood Spot

Molecular Detection

Human immunodeficiency virus (HIV)

National Laboratory for HIV Reference Services

• Acquired immunodeficiency syndrome (AIDS)

HIV: Full dried blood spot card; 5 full circles optimal, <5 circles limits testing.

Contact NLHRS for specimens other than those listed above.

Note: Specimens categorized as “other” are not subject to accreditation by the Standards Council of Canada for the specific scopes of accreditation published on www.scc.ca.

See specimen above.

See Dried Blood Spot Collection, Packaging & Storage Guidelines.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Please provide risk factors.

NLHRS DBS Requisition: HIV, HCV, Syphilis & HBsAg

See Dried Blood Spot Collection, Packaging & Storage Guidelines.

Varies for each test method, contact NLHRS for further information.

Varies for each test method, contact NLHRS for further information.

1. Standard Council of Canada. SCC accredits first medical laboratory under new national program.  2008. http://www.scc.ca/en/news-events/news/2008/scc-accredits-first-medical-laboratory-under-new-national-program
2. S.Lavoie, D. Caswell, M.J. Gill, K. Kadkhoda, C.L Charlton, P.N. Levett, T.Hatchette, R. Garceau, J. Maregmen, T. Mazzulli, R. Needle, K. Kadivar, J. Kim. Heterophilic interference in specimens yielding false-reactive results on the Abbott 4th generation ARCHITECT HIV Ag/Ab Combo Assay. Journal of Clinical Virology. 2018;104: 23-28
3. Malloch L, Kadivar K, Putz J, Levett PN, Tang J, Hatchette TF, Kadkhoda K, Ng D, Ho J, Kim J. Comparative Evaluation of the Bio-Rad Geenius HIV-1/2 Confirmatory Assay and the Bio-Rad Multispot HIV-1/2 Rapid Test as an Alternative Differentiation Assay for CLSI M53 Algorithm-I. Journal of Clinical Virology. 2013;58, Supplement 1(0):e85-e91. http://www.sciencedirect.com/science/article/pii/S1386653213003326
4. Kadivar K, Malloch L, Adonsou-Hoyi Y, Ng D, Lavoie S, Pulido K, Kim J. Would CLSI M53-A Have Helped in the Diagnosis of HIV in Canada? Results of the Performance of Canadian Laboratories Participating in a Recent NLHRS Proficiency Testing Panel Containing HIV-1 Antigen Positive (Antibody Negative) and HIV-2 Samples. Journal of Clinical Virology. 2013;58(1):303-305. http://www.sciencedirect.com/science/article/pii/S1386653213001303

Pathogen Safety Data Sheets: Infectious Substances – Human immunodeficiency virus (HIV)

Biosafety Directive for Human Immunodeficiency Virus (HIV) and Human T-cell Lymphotropic Virus Type 1 (HTLV-1)

Human Immunodeficiency Virus - HIV Screening and Testing Guide

Canadian Guidelines on Sexually Transmitted and Blood-Borne Infections

National case definition: Acquired immune deficiency syndrome (AIDS)

HIV and AIDS: For health professionals

National case definition: Human immunodeficiency virus (HIV)

HIV and AIDS