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Detection of IgG Antibodies Directed Towards Powassan Virus by ELISA

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Requisition Forms

Reference Details

Description:

Serological detection of IgG antibodies directed towards Powassan virus by Euroimmun Anti-Powassan Virus ELISA (IgG).

Test Category:
Serology
Pathogen:
Powassan virus
Illnesses and Diseases:
  • Powassan encephalitis
Specimen:

Serum. Minimum volume of 250 µl required.

Collection Method:

2 mL screw cap tubes.

Specimen Processing, Storage and Shipping:

Store samples refrigerated until shipped for testing. Ship samples on a cold pack, or with wet or dry ice.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Patient Criteria:

Suspected infection with Powassan virus and exposure to relevant arthropod.

Accompanying Documentation:

Completed Viral Zoonoses requisition including sender laboratory name, address and telephone number.  Patient name and / or identifier (specimen reference number), date of birth, test(s) requested, collection date of specimen, date of onset of symptoms, and clinical and travel history of patient. 

Comments:

All diagnostic samples are tested by both IgM and IgG ELISA to increase the clinical sensitivity.

This test is considered investigational as it has not been extensively validated or verified.

Methods and Interpretation of Results:

Testing is performed, in whole or in part, using a “Research Use Only” testing kit which has not been fully validated/verified due to a lack of well-characterized panel.    

The detection of IgG antibodies in a single serum sample is indicative of past or present exposure to this virus. A 4 fold rise or greater in neutralizing antibody titre, or seroconversion in paired sera, is required to document a ’confirmed case’ of infection.

IgM can persist in serum for up to a year or more after arbovirus exposure.  Thus, detection of IgM by itself is not sufficient for confirmation of acute infection, but is consistent with an exposure at an undetermined time.

Isolation of an arbovirus or detection of nucleic acid by real-time RT-PCR in a clinical specimen provides clear evidence of infection associated with the current clinical illness.

Turnaround Time:

14 Calendar days.

Contact:
Phone #: (204) 789-7037
Fax: (204) 789-2018
References:
  1. Euroimmun Medizinische Labordiagnostika AG. Anti-Powassan Virus ELISA (IgG) test Instructions Version 22/11/2017.
  2. Johnson, A.J., Martin, D.A., Karabatsos, N., and Roehrig, J.T. Detection of anti-arboviral immunoglobulin G by using a monoclonal antibody-based capture enzyme-linked immunosorbent assay. J. Clin. Micro. 38; 1827-1831.
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