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Whole Genome Sequencing (WGS)

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Requisition Forms

Reference Details

Description:

Molecular typing of Burkholderia mallei strains using Whole Genome Sequencing data.

Test Category:
Molecular Differentiation
Pathogen:
Burkholderia mallei
Illnesses and Diseases:
  • Glanders
Specimen:

Pure bacterial culture

Collection Method:

Pure bacterial culture – plate or swab

Specimen Processing, Storage and Shipping:

Store bacterial cultures refrigerated until shipped for testing.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Patient Criteria:

Confirmed Burkholderia mallei infection. 

Accompanying Documentation:

Completed Bioforensics Assay Development and Diagnostics requisition including sender name, address and telephone number. Patient name or identifier (referring lab #), date of birth, suspected exposure, test(s) requested, clinical history, travel history, type of specimen and date collected.

Comments:

N/A

Methods and Interpretation of Results:

The phylogenetic relationships between strains are determined using Whole Genome Sequencing data based on single base pair variants (SNVs) at specific genomic locations.

Turnaround Time:

14 calendar days for a final result.

Contact:
Phone #: (204) 784-5928, 1-877-212-6108 emergency
Fax: (204) 789-5009
References:
  1. Petkau, A, et al. 2017. “SNVPhyl: A Single Nucleotide Variant Phylogenomics Pipeline for Microbial Genomic Epidemiology.” Microb Genom, Jun 8;3(6):e000116. doi: 10.1099/mgen.0.000116
Guidelines:
Related Information: