Whole Genome Sequencing (WGS)

Molecular typing of Burkholderia pseudomallei strains using Whole Genome Sequencing data.

• Melioidosis

Pure bacterial culture

Pure bacterial culture – plate or swab

Store bacterial cultures refrigerated until shipped for testing.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Confirmed Burkholderia pseudomallei infection. 

Completed Bioforensics Assay Development and Diagnostics requisition including sender name, address and telephone number. Patient name or identifier (referring lab #), date of birth, suspected exposure, test(s) requested, clinical history, travel history, type of specimen and date collected.

N/A

The phylogenetic relationships between strains are determined using Whole Genome Sequencing data based on single base pair variants (SNVs) at specific genomic locations.The phylogenetic relationships between strains are determined using Whole Genome Sequencing data based on single base pair variants (SNVs) at specific genomic locations.

14 calendar days for a final result.

1. Petkau, A, et al. 2017. “SNVPhyl: A Single Nucleotide Variant Phylogenomics Pipeline for Microbial Genomic Epidemiology.” Microb Genom, Jun 8;3(6):e000116. doi: 10.1099/mgen.0.000116

Burkholderia (Pseudomonas) pseudomallei - Material Safety Data Sheets (MSDS)