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Molecular Determination of Neisseria gonorrhoeae Multi-antigen Sequence Type (NG-MAST) using Gonorrhea-positive nucleic acid amplification test specimens (NAATs)

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Reference Details


Determination of Neisseria gonorrhoeae Multi-antigen Sequence Type (NG-MAST) on nucleic acid amplification test specimens (NAATs)

Test Category:
Molecular Typing
Neisseria gonorrhoeae
Illnesses and Diseases:
  • Gonorrhea

Gonorrhoea positive specimens in APTIMA or Roche Cobas buffer, including urine, urethral/urogenital swabs, vaginal swabs, and rectal swabs, or DNA extracts. Neat urine and pharyngeal swabs are not recommended. Other specimen types may be accepted with proper rationale for testing or by prior approval from the Streptococcus and STI Unit. The minimum volume required is 1mL. Submit only one GC NAAT per case unless related to a treatment failure investigation.

Specimens may be subject to rejection if they are not the appropriate sample type, have insufficient volume, or are not accompanied by relevant patient information.


Collection Method:


Specimen Processing, Storage and Shipping:

Store samples refrigerated or frozen until shipped for testing.  Ship frozen samples on dry ice and refrigerated samples on cold packs.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.


Patient Criteria:

Infection with N. gonorrhoeae. 

Accompanying Documentation:

Completed NML GC NAAT requisition form with sender lab name, address, telephone number and email address.  All patient information must be filled out including patient identified (specimen reference #), date of birth and sex, testing platform, date collected, isolation site and test requested. Urgent specimens must be stated as such on the requisition. The lab should be informed of urgent samples prior to receiving them.



Methods and Interpretation of Results:

DNA from all gonorrheal samples received at the NML for PCR testing is initially extracted using a commercially-available kit. Results are based upon PCR assays that amplify the sequences of 2 genes, porB and tbpB, used in the determination of NG-MAST sequence types. The sequence types are analyzed and the combination of the porB and tbpB alleles determine the NG-MAST type for the specimen (1, 2). Results are for surveillance purposes only.

Turnaround Time:


Phone #: (204) 789-6063; STI: (204) 784-5995
Fax: (204) 789-2140
  1. Martin IMC, Ison CA, Foreman E, Hall V, Nesbitt A, Forster G, and Ison AC. Non-cultural detection and molecular genotyping of Neisseria gonorrhoeae from a piece of clothing. J Med Micro 2007;56:487-490
  2. Martin IMC, Ison CA, Aanensen DM, Fenton KA, Spratt BG. Rapid sequence-based identification of gonococcal transmission clusters in a large metropolitan area. J Infect Dis 2004;189:1497-1505.
  3. Peterson SW, Martin I, Demczuk W, Barairo N, Naidu P, Lefebvre B, Allen V, Hoang L, Hatchette TF, Alexander D, Tomas K, Trubnikov M, Wong T, Mulvey MR. Multiplex real-time PCR assays for the prediction of cephalosporin, ciprofloxacin and azithromycin antimicrobial susceptibility of positive Neisseria gonorrhoeae nucleic acid amplification test samples. J Antimicro Chemo. 2020; 75: 3485-3490.
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