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Identification of Aerobic Actinomycetes Species

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Requisition Forms

Reference Details


Identification of aerobic actinomycetes isolates to the genus or species level as per ‘LPSN (List of Prokaryotic names with Standing in Nomenclature, author:  J.P. Euzeby).’  The testing is conducted using a combination of MALDI-TOF (matrix-assisted laser desorption/ionization – Time Of Flight) Mass Spectrometry* and/or DNA sequence-based methodologies.

Test Category:
Aerobic Actinomycetes
Illnesses and Diseases:
  • Various

Pure cultures of isolates recovered from normally sterile sites and serious wound infections.

Collection Method:


Specimen Processing, Storage and Shipping:

Ship all cultures at room temperature (DO NOT freeze) for overnight delivery, and prior to Wednesday each week to ensure receipt by Friday.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.


Patient Criteria:


Accompanying Documentation:

Notification of sample submission must be emailed or faxed prior to shipping isolates. Samples should be shipped attention: Melissa Rabb, Head, Reference and Diagnostic Services, NRCM/NML at 204-789-6081/204-789-6038.  A requisition for the NRCM must be completed and signed off by the supervisor/designate of the submitting laboratory and include the source of specimen, patient gender, date of birth, clinical history, submitting laboratory identifier and submitter information.  Please also include isolate characteristics:  microscopy, pigmentation, culture characteristics, growth rate/temperature, and other identification.  Requested testing for urgent submissions should be accompanied by a justification.


Cultures will be rejected if appropriate documentation and justification is incomplete or missing. 

Methods and Interpretation of Results:

Species identification will be determined using MALDI-TOF, 16S rRNA and/or hsp65 gene fragment sequencing. The identification will be determined by comparison of sequencing data to NCBI BLAST database or Bruker MALDI-TOF database.   

Turnaround Time:

Routine testing from culture: 10 calendar days from the date of specimen receipt.

Phone #: (204) 789-2136 or (204) 789-6038
Fax: (204) 789-2036
  1. Clinical and Laboratory Standards Institute. 2018. Susceptibility Testing of Mycobacteria, Nocardia spp., and Other Aerobic Actinomycetes; Approved Standard-Third Edition. M24-Ed3. Clinical and Laboratory Standards Institute, Wayne, Pennsylvania.
  2. Clinical and Laboratory Standards Institute. 2018. Performance Standards for Susceptibility Testing of Mycobacteria, Nocardia spp., and Other Aerobic Actinomycetes; First Edition. M62-Ed1. Clinical and Laboratory Standrads Institute, Wayne, Pennsylvania.
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