Isolation of Severe Fever with Thrombocytopenia Syndrome Virus

Detection of SFTSV virus infection by isolation of virus.

Isolation

Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV)

Special Pathogens

• Severe fever with thrombocytopenia syndrome

Whole blood, serum, CSF, other bodily fluids (minimum sample 0.5 mL) and fresh frozen tissues.

Sang total : Prélever dans des tubes avec EDTA, héparine ou citrate. Peut être envoyé en aliquotes dans des tubes stériles à bouchon à vis de 1,5 à 2,0 ml.

Sérum : Recueillir dans des tubes à sérum (avec bouchon rouge) ou dans des tubes séparateurs de sérum (TSS). La centrifugation des tubes à sérum n’est pas nécessaire et doit en fait être évitée.

LCR et autres liquides organiques : Prélever les échantillons dans des tubes stériles à bouchon à vis de 1,5 à 2,0 ml.

Tissus : Les tissus frais congelés doivent être placés dans des récipients en plastique.

Store samples in refrigerator or frozen until being shipped for testing. Avoid freezing whole blood samples. Tissues should be shipped frozen. Ship frozen specimens on dry ice and refrigerated specimens on wet ice.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Symptomatic and travel history to endemic regions.

Completed Special Pathogens requisition including sender name, address and telephone number. Patient information including name or identifier, date of birth, and all relevant patient history including clinical diagnosis, underlying diseases, exposure risk and travel history. Indicate type of specimen collected and all specimen information. Select all required tests. If possible, attach lab results that have already been done at local or provincial laboratories.

All patient history must be included; sample without adequate patient history to justify testing are subject to rejection.

Isolation is a non-routine test for investigational purposes.

For diagnosis of SFTSV infection to be confirmed, one or more of the following diagnostic markers must be positive: (i) presence of SFTSV RNA by PCR, (ii) isolation of SFTS virus.

14 calendar days.

1. https://www.nejm.org/doi/10.1056/NEJMoa1010095?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dwww.ncbi.nlm.nih.gov