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Molecular Detection of Bas-Congo virus

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Requisition Forms

Reference Details

Description:

Detection of BASV virus infection by PCR amplification of BASV virus specific genome.

Test Category:
Molecular Detection
Pathogen:
Bas-Congo virus
Illnesses and Diseases:
  • Acute hemorrhagic fever
Specimen:

Whole blood, serum, CSF, other bodily fluids (minimum sample 0.5 mL) and fresh frozen tissues. Formalin-fixed or paraffin-embedded tissues.

Collection Method:

Whole blood: collect in EDTA, heparin or citrate tubes, can submit as aliquots in sterile 1.5-2.0 mL screw cap tubes.

Serum: collect in serum (red top) or serum separator tubes (SST), centrifugation of serum tubes is not needed and should be avoided.

CSF and other bodily fluids:  Collect in sterile 1.5-2.0 mL screw cap tubes.

Tissues: Fresh frozen tissues should be placed in plastic containers. Tissues that are formalin-fixed should also be sent in plastic containers and clearly identified as being in formalin. Paraffin-embedded tissues can be sent as entire blocks or four to six 10 uM sections in a plastic tube or vial.

Specimen Processing, Storage and Shipping:

Store samples in refrigerator or frozen until being shipped for testing. Avoid freezing whole blood samples. Tissues should be shipped frozen. Ship frozen specimens on dry ice and refrigerated specimens on wet ice. Formalin-fixed samples can be sent at room temperature.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Patient Criteria:

Symptomatic and travel history to endemic regions.

Accompanying Documentation:

Completed Special Pathogens requisition including sender name, address and telephone number. Patient information including name or identifier, date of birth, and all relevant patient history including clinical diagnosis, underlying diseases, exposure risk and travel history. Indicate type of specimen collected and all specimen information. Select all required tests. If possible, attach lab results that have already been done at local or provincial laboratories.

Comments:

All patient history must be included; sample without adequate patient history to justify testing are subject to rejection.

Non-routine test for investigational purposes.

Methods and Interpretation of Results:

For diagnosis of BASV infection to be confirmed, one or more of the following diagnostic markers must be positive: (i) presence of BASV RNA by PCR, (ii) isolation of BAS virus.

Turnaround Time:

14 calendar days.

Contact:
Phone #: (204) 789-6019 or (204) 789-7032*
Fax: (204) 789-2140
Fact Sheets:
Guidelines:
Related Information: