Antimicrobial Susceptibility Testing (AST)

<<Return to Laboratory

Accredited by the Standards Council of Canada to Laboratory no. 594 - CAN-P-4E (ISO/IEC 17025)

Requisition Forms

Reference Details

Description:

Antimicrobial Susceptibility Testing (AST) broth microdilution method as described by the Clinical and Laboratory Standards Institute (CLSI) guidelines.

Test Category:
Susceptibility Testing
Pathogen:
Burkholderia pseudomallei
Illnesses and Diseases:
  • Glanders
Specimen:

Actively growing, pure, fresh bacterial culture.

Collection Method:

Pure bacterial culture – plate or swab.

Specimen Processing, Storage and Shipping:

Store bacterial cultures refrigerated until shipped for testing. 

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Confirmed Burkholderia pseudomallei infection.

Accompanying Documentation:

Completed Bioforensics Assay Development and Diagnostics requisition including sender name, address and telephone number. Patient name or identifier (referring lab #), date of birth, suspected exposure, test(s) requested, clinical history. Type of specimen and date collected. If possible, include the antibiotics administered and travel history. 

Comments:

All patient history must be included.

Methods and Interpretation of Results:

Broth microdilution using accredited In-House prepared panels. Interpretations are based on the most recent CLSI guidelines.

Turnaround Time:

14 calendar days for a final result.

Contact:
Phone: (204) 784-5928 or 1-877-212-6108
Fax: (204) 789-5009
References:
  1. Canadian Biosafety Standard for Facilities Handling or Storing Human and Terrestrial Animal Pathogens and Toxins; Canadian Biosafety Standard, Second Edition, 2015
  2. CLSI. Performance Standards for Antimicrobial Susceptibility Testing. 29th ed. CLSI supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute; 2019.
  3. CLSI.  Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically. 11th ed. CLSI supplement M07. Wayne, PA: Clinical and Laboratory Standards Institute; 2018.
  4. CLSI. Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria. 3rd ed. CLSI guidelines M45. Wayne, PA: Clinical and Laboratory Standards Institute; 2016.
Guidelines:
Related Information: