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Antimicrobial Susceptibility Testing (AST)

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Accredited by the Standards Council of Canada to Laboratory no. 594 (ISO/IEC 17025)

Requisition Forms

Reference Details

Description:

Antimicrobial Susceptibility Testing (AST) broth microdilution method as described by the Clinical and Laboratory Standards Institute (CLSI) guidelines.

Test Category:
Susceptibility Testing
Pathogen:
Bacillus anthracis
Illnesses and Diseases:
  • Anthrax
Specimen:

Actively growing, pure, fresh bacterial culture.

Collection Method:

Pure bacterial culture – plate or swab.

Specimen Processing, Storage and Shipping:

Store bacterial cultures refrigerated until shipped for testing. 

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

 

Patient Criteria:

Confirmed Bacillus anthracis infection.

Accompanying Documentation:

Completed Bioforensics Assay Development and Diagnostics requisition including sender name, address, and telephone number. Patient name or identifier (referring lab #), date of birth, suspected exposure, test(s) requested, clinical history, type of specimen and date collected. If possible, include the antibiotics administered and travel history. 

 

Comments:

All patient history must be included. Please notify the laboratory prior to submission of specimen.

Methods and Interpretation of Results:

Broth microdilution using accredited in-house prepared antibiotic panels. Interpretations are based on the most recent CLSI guidelines.

 

Turnaround Time:

14 calendar days for a final result.

Contact:
Phone #: (204) 784-5928 or 1-877-212-6108
Fax: (204) 789-5009
References:
  1. Canadian Biosafety Standard for Facilities Handling or Storing Human and Terrestrial Animal Pathogens and Toxins; Canadian Biosafety Standard, Second Edition, 2015
  2. CLSI. Performance Standards for Antimicrobial Susceptibility Testing. 33rd ed. CLSI supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute; 2023.
  3. CLSI.  Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically. 11th ed. CLSI supplement M07. Wayne, PA: Clinical and Laboratory Standards Institute; 2018.
  4. CLSI. Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria. 3rd ed. CLSI guidelines M45. Wayne, PA: Clinical and Laboratory Standards Institute; 2016.
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