Single Nucleotide Polymorphisms (SNPs)

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Accredited by the Standards Council of Canada to Laboratory no. 594 - CAN-P-4E (ISO/IEC 17025).

Requisition Forms

Reference Details

Description:

Molecular typing of Yersinia pestis strains to determine genetic variations between members of a species.

Test Category:
Molecular Differentiation
Pathogen:
Yersinia pestis
Illnesses and Diseases:
  • Plague
Specimen:

Pure bacterial culture.

Collection Method:

Pure bacterial culture – plate or swab.

Specimen Processing, Storage and Shipping:

Store bacterial cultures refrigerated until shipped for testing

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Confirmed Yersinia pestis infection.

Accompanying Documentation:

Completed Bioforensics Assay Development and Diagnostics requisition including sender name, address and telephone number.  Patient name or identifier (referring lab #), date of birth, suspected exposure, test(s) requested, clinical history.  Type of specimen and date collected.  If possible, include the antibiotics administered and travel history. 

Comments:

N/A

Methods and Interpretation of Results:

The SNP pattern will be determined through PCR, looking for single base pair mutations at a specific locus which usually consists of two alleles. 

Turnaround Time:

14 calendar days for a final result.

Contact:
Phone: (204) 784-5928 or 1-877-212-6108
Fax: (204) 789-5009
References:
  1. Vogler AJ et al. Biotechniques. 2008 February ; 44(2): 201–207 Assays for the rapid and specific identification of North American Yersinia pestis and the common laboratory strain CO92.
  2. Andrea Ciammaruconi et al. Diagnostic Microbiology and Infectious Disease 65 (2009) 7–13 A rapid allele variant discrimination method for Yersinia pestis strains based on high-resolution melting curve analysis.
  3. Giovanna Morelli et al. Nat Genet. 2010 December ; 42(12): 1140–1143. doi:10.1038/ng.705. Yersinia pestis genome sequencing identifies patterns of global phylogenetic diversity.
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