Single Nucleotide Polymorphisms (SNPs)
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Requisition Forms
Reference Details
Molecular typing of Yersinia pestis strains to determine genetic variations between members of a species.
- Plague
Pure bacterial culture.
Pure bacterial culture – plate or swab.
Store bacterial cultures refrigerated until shipped for testing
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
For additional guidance on the transport of infectious substances in other languages, please click on the link below.
http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/
Confirmed Yersinia pestis infection.
Completed Bioforensics Assay Development and Diagnostics requisition including sender name, address and telephone number. Patient name or identifier (referring lab #), date of birth, suspected exposure, test(s) requested, clinical history. Type of specimen and date collected. If possible, include the antibiotics administered and travel history.
N/A
The SNP pattern will be determined through PCR, looking for single base pair mutations at a specific locus which usually consists of two alleles.
14 calendar days for a final result.
- Vogler AJ et al. Biotechniques. 2008 February ; 44(2): 201–207 Assays for the rapid and specific identification of North American Yersinia pestis and the common laboratory strain CO92.
- Andrea Ciammaruconi et al. Diagnostic Microbiology and Infectious Disease 65 (2009) 7–13 A rapid allele variant discrimination method for Yersinia pestis strains based on high-resolution melting curve analysis.
- Giovanna Morelli et al. Nat Genet. 2010 December ; 42(12): 1140–1143. doi:10.1038/ng.705. Yersinia pestis genome sequencing identifies patterns of global phylogenetic diversity.