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Whole Genome Sequencing (WGS) and Single Nucleotide Polymorphisms (SNPs)

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Accredited by the Standards Council of Canada to Laboratory no. 594 (ISO/IEC 17025).

Requisition Forms

Reference Details

Description:

Multi-faceted approach to the molecular typing of Yersinia pestis strains.

WGS Test: Molecular typing of Yersinia pestis strains using Whole Genome Sequencing data.

SNP Test: Molecular typing Yersinia pestis strains to determine genetic variations between members of a species, using single nucleotide polymorphisms.

Test Category:
Molecular Differentiation
Pathogen:
Yersinia pestis
Illnesses and Diseases:
  • Plague
Specimen:

Pure bacterial culture.

Collection Method:

Pure bacterial culture – plate or swab.

Specimen Processing, Storage and Shipping:

Store bacterial cultures refrigerated until shipped for testing

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

 

Patient Criteria:

Confirmed Yersinia pestis infection.

Accompanying Documentation:

Completed Bioforensics Assay Development and Diagnostics requisition including sender name, address and telephone number.  Patient name or identifier (referring lab #), date of birth, suspected exposure, test(s) requested, clinical history.  Type of specimen and date collected.  If possible, include the antibiotics administered and travel history. 

Comments:

N/A

Methods and Interpretation of Results:

WGS Test: The phylogenetic relationships and subtype strains are determined using Whole Genome Sequencing data based on single base pair variants (SNVs) at specific genomic locations.

SNP Test: The SNP pattern is determined through PCR, looking for single base pair mutations at a specific locus which usually consists of two alleles. 

Turnaround Time:

14 calendar days for a final result.

Contact:
Phone #: (204) 784-5928 or 1-877-212-6108
Fax: (204) 789-5009
References:
  1. Vogler AJ et al., 2008. Assays for the rapid and specific identification of North American Yersinia pestis and the common laboratory strain CO92 Biotechniques. February ; 44(2): 201–207
  2. Ciammaruconi A et al., 2009. A rapid allele variant discrimination method for Yersinia pestis strains based on high-resolution melting curve analysis. Diagnostic Microbiology and Infectious Disease 65; 7–13
  3. Morelli G et al., 2010. Yersinia pestis genome sequencing identifies patterns of global phylogenetic diversity.Nat Genet. December; 42(12): 1140–1143. doi:10.1038/ng.705.
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