Molecular Detection of Mutations Associated with Antimicrobial Resistance

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Requisition Forms

Reference Details

Description:

Detection of mutations associated with antimicrobial resistance in Mycoplasma genitalium by PCR.

Test Category:
Susceptibility Testing
Pathogen:
Mycoplasma genitalium
Illnesses and Diseases:
  • Non-gonococcal urethritis (NGU)
Specimen:

Urethral/Urogenital swab, vaginal swab, rectal swab, urine, DNA extracts. Other specimen types may be accepted with proper rationale for testing or by prior approval from the Streptococcus and STI Unit.

Collection Method:

Sterile cotton swab or Dacron polyester swab or flocked nylon swab.

Specimen Processing, Storage and Shipping:

Place swabs in at least 1 mL appropriate transport medium (such as Universal Transport Medium).   Store samples refrigerated or frozen until shipped for testing.  Ship frozen samples on dry ice and refrigerated samples on cold packs.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Infected with Mycoplasma genitalium.

Accompanying Documentation:

Streptococcus and STI Unit requisition form, clearly specify organism testing request on the requisition form. Append all relevant clinical background information and testing performed.

Comments:

N/A

Methods and Interpretation of Results:

DNA from all urogenital mycoplasma samples received at the NML for PCR testing is initially extracted using a commercially-available kit. Results are based upon PCR assays that detect mutations in 23S ribosomal RNA (associated with azithromycin resistance) and parC and gyrA (associated with moxifloxacin resistance).  The presence of the specific mutations predicts resistance to the associated antimicrobial, but, as with any laboratory test, the results of the test should be interpreted with consideration of all of the laboratory and clinical findings available.

Turnaround Time:

14 calendar days. Urgent specimens will be granted priority status, and completed as soon as possible.

Contact:
Phone: Office: (204) 789-6063
Fax: (204) 789-5012
References:
  1. Jensen JS. Protocol for the detection of Mycoplasma genitalium by PCR from clinical specimens and subsequent detection of macrolide resistance-mediating mutations in region V of the 23S rRNA gene. Methods in Molecular Biology. 2012; 903:129-139.
  2. Shimada Y, Deguchi T, Nakane K, Masue T, et al. Emergence of clinical strains of Mycoplasma genitalium harbouring alterations in ParC associated with fluorouinolone resistance. 2010. Int J Antimicrob Agents. Sep;36(3):255-8.
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