PCR

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Reference Details

Description:

Klebsiella species PCR test.

Test Category:
Molecular Differentiation
Pathogen:
Klebsiella spp
Illnesses and Diseases:
  • Pneumonia
  • Bacteremia
  • Meningitis
  • Urinary tract infection
  • Wound
Specimen:

Pure culture.

Collection Method:

N/A

Specimen Processing, Storage and Shipping:

Send cultures as growth on culture medium or in transport medium.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.  http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

N/A

Accompanying Documentation:

Completed Antimicrobial Resistance and Nosocomial Infections Requisition.

Comments:

The organism(s) should have been identified as a putative Klebsiella pneumoniae or Klebsiella oxytoca by the submitting laboratory. This test will specifically identify K. pneumoniae pneumoniae, K. variicola, K. quasipneumoniae, and K. oxytoca.

Methods and Interpretation of Results:

PCR based method. Results compared to known standards.

Turnaround Time:

6 calendar days.

Contact:
Phone: (204) 789-5000
Fax: (204) 789-5020
References:
  1. Garza-Ramos U, Silva-Sanchez J, Martinez-Romero E, Tinoco P, Pina-Gonzales M, Barrios H, Martinez-Barnetche J, Gomez-Barreto, Tellez-Sosa J. Development of a multiplex-PCR probe system for the proper identification of Klebsiella variicola. BMC Microbiology 2015; 15:64.
  2. Fonesca EL, da Veiga Ramos N, Andrade BGN, Morais LLCS, Marin MFA, Vicente AC. A one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine. Diag Microbiol Infect Dis 2017; 87:315-317.
  3. Kotunovych G, Lytvynenko T, Negrutska V, Lar O, Brisse S, Kozyrovska N. Identification of Klebsiella oxytoca using a specific PCR assay targeting the polygalcturonase pehX gene. Res Microbiol 2003 154:587-92.
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