ELISA Test - IgG

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Reference Details

Description:

ELISA Test IgG to Orientia tsutsugamushi.

Test Category:
Serology
Pathogen:
Orientia tsutsugamushi
Illnesses and Diseases:
  • Scrub typhus
Specimen:

Serum sample.  Minimum volume required – 0.5 mL.

Note:  0.5 ml is sufficient volume for all requested Rickettsial testing on one sample.

Collection Method:

Submit in a sterile microcentrifuge tube.

Specimen Processing, Storage and Shipping:

Store samples refrigerated or frozen until shipped for testing.  Ship frozen on dry ice or on wet ice.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Suspected scrub typhus infection; history of travel to an endemic region. Scrub typhus has almost exclusively been reported from Pakistan in the west to eastern Russia in the east to Australia in the south.

Accompanying Documentation:

Completed Rickettsia and Related Zoonotic Diseases requisition including sender name, address and telephone number.  Patient name or identifier (referring lab #), date of birth, suspected exposure, test(s) requested and clinical history. Also include type of specimen, date collected, travel history and if possible, antibiotics administered.  

Note: A travel history compatible with the distribution of Orientia tsutsugamushi is required before testing will be performed.

Comments:

N/A

Methods and Interpretation of Results:

Serum samples are tested at a 1:100 dilution in a qualitative, membrane based immunoassay for the detection of IgG antibodies to Orientia tsutsugamushi.

Turnaround Time:

15 calendar days.

Contact:
Phone: (204) 789-7037
Fax: (204) 789-2018
References:
Fact Sheets:
Guidelines:
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