Resistance Mechanisms

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Requisition Forms

Reference Details

Description:

Beta-lactamase or carbapenemase resistance mechanisms are tested by PCR.

Test Category:
Molecular Detection
Pathogen:
Gram negative organisms
Illnesses and Diseases:
  • Pneumonia
  • Bacteremia
  • Meningitis
  • Urinary tract infection
  • Wound
Specimen:

Pure culture.

Collection Method:

N/A

Specimen Processing, Storage and Shipping:

Send cultures as growth on culture medium or in transport medium.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.  http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

N/A

Accompanying Documentation:

Completed Antimicrobial Resistance and Nosocomial Infections Requisition.

Comments:

Gram negative organisms may include but are not limited to: E. coli, Klebsiella sp., Acinetobacter sp., Pseudomonas sp.

Methods and Interpretation of Results:

PCR based method. Results are compared to known standards.

Turnaround Time:

6 calendar days.

Contact:
Phone: (204) 789-5000
Fax: (204) 789-5020
References:
  1. Mataseje LF, et al 2016. Carbapenemase-Producing Enterobacteriaceae in Canada: Results from the Canadian Nosocomial Infection Surveillance Program, 2010-2014. Antimicrob Agents Chemother. pii: AAC.01359-16.
  2. Liu Y-Y, Wang Y, Walsh TR, Yi L-X, Zhang R, Spencer J, Doi Y, Tian G, Dong B, Hunag X, Yu L-F, Gu D, Ren H, Chen X, Lv L, He D, Zhou H, Liang Z, Liu J-H, Shen J. 2015. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular study. Lancet Infect Dis; dx.doi.org/10.1016/S1473-3099(15)00424-7.
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