Resistance Mechanisms
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Beta-lactamase or carbapenemase resistance mechanisms are tested by PCR.
- Pneumonia
- Bacteraemia
- Meningitis
- Urinary tract infection
- Wound
Pure culture.
N/A
Send cultures as growth on culture medium or in transport medium. Culture vessel should be leak-proof or sealed appropriately.
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
N/A
Completed Antimicrobial Resistance and Nosocomial Infections Requisition.
Gram negative organisms may include but are not limited to: most Enterobacterales, Acinetobacter sp., and Pseudomonas sp.
PCR based method. Results are compared to known standards.Routine beta-lactamases tested: blaTEM, blaSHV, blaCTX-M, blaOXA-1,. Routine AmpC tested: blaCMY-2-type. Routine carbapenemase variant-types tested: blaKPC, blaIMP, blaVIM, blaNDM, blaGES, blaOXA-48, blaFRI, blaSME, blaNMC/IMI. In addition, the following colistin resistance genes are tested: mcr1/2, mcr-3.
6 calendar days.
- Mataseje LF, et al 2016. Carbapenemase-Producing Enterobacteriaceae in Canada: Results from the Canadian Nosocomial Infection Surveillance Program, 2010-2014. Antimicrob Agents Chemother. pii: AAC.01359-16.
- Liu Y-Y, Wang Y, Walsh TR, Yi L-X, Zhang R, Spencer J, Doi Y, Tian G, Dong B, Hunag X, Yu L-F, Gu D, Ren H, Chen X, Lv L, He D, Zhou H, Liang Z, Liu J-H, Shen J. 2015. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular study. Lancet Infect Dis; dx.doi.org/10.1016/S1473-3099(15)00424-7.