VISA/hVISA Confirmation
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Confirmation of vancomycin intermediate Staphylococcus aureus (VISA) or heterogeneous vancomycin intermediate Staphylococcus aureus (hVISA) by Etest.
- Necrotizing pneumonia
- Skin and soft tissue infections
Pure culture.
N/A
Send cultures as growth on culture medium or in transport medium. Culture vessel should be leak-proof or sealed appropriately.
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
N/A
Completed Antimicrobial Resistance and Nosocomial Infections Requisition.
As this is not the gold standard test for determination of VISA status (PAP-AUC), this combination of tests only indicates if the organism may be a hVIS/VISA if the vancomycin MIC by standard Etest is <=2 mg/L.
A combination of standard vancomycin Etest MIC determination and macro & GRD Etests are used to indicate if the organism is a probable hVISA or VISA or not.
8 calendar days.
- Walsh TR1, Bolmström A, Qwärnström A, Ho P, Wootton M, Howe RA, MacGowan AP, Diekema D. 2001. Evaluation of current methods for detection of staphylococci with reduced susceptibility to glycopeptides. J Clin Microbiol. Jul;39(7):2439-44.
- Yusof A, et al. 2008. Evaluation of a New Etest Vancomycin-Teicoplanin strip for detection of glycopeptide-intermediate Staphylococcus aureus (GISA), in particular heterogeneous GISA. J Clin Microbiol 46(9) 3042-3047.